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  Indian J Med Microbiol
 

Figure 5: Effects of Akt inhibition on methanolic extract of Dictamnus dasycarpus (MEDD)-induced apoptosis in AGS cells. Cells were pretreated with Akt inhibitor (LY294002 (20 μM)) for 1 h and then treated with MEDD (280 μg/ml) for 24 h. (a) Cells were collected and deoxyribonucleic acid contents were analyzed by flow cytometry. (b) Equal amounts of cell lysates were extracted, separated in SDS-polyacrylamide gels and transferred to nitrocellulose membranes, which were probed with the indicated antibodies. Proteins were visualized using an enhanced chemiluminescence detection system. Actin was used as an internal control

Figure 5: Effects of Akt inhibition on methanolic extract of <i>Dictamnus dasycarpus</i> (MEDD)-induced apoptosis in AGS cells. Cells were pretreated with Akt inhibitor (LY294002 (20 μM)) for 1 h and then treated with MEDD (280 μg/ml) for 24 h. (a) Cells were collected and deoxyribonucleic acid contents were analyzed by flow cytometry. (b) Equal amounts of cell lysates were extracted, separated in SDS-polyacrylamide gels and transferred to nitrocellulose membranes, which were probed with the indicated antibodies. Proteins were visualized using an enhanced chemiluminescence detection system. Actin was used as an internal control