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  Indian J Med Microbiol
 

Figure 4: Effects of Akt inhibition on methanolic extract of Dictamnus dasycarpus (MEDD)-induced AGS cell death. (a) Cells were pretreated with the indicated mitogen-activated protein kinases inhibitors (SB203580 (20 μM), SP600125 (20 μM), or PD98059 (50 μM)) for 1 h and then treated with MEDD (350 μg/ml) for 24 h. (b) cells were pretreated with the Akt inhibitor (LY294002 (20 μM)) for 1 h and then treated with MEDD (280 μg/ml) for 24 h. Cell viabilities were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium assay. Results are the means ± standard deviations of two different experiments. *P < 0.05 versus untreated cells; #P < 0.05 versus MEDD-treated cells; n.s.: Not significant versus MEDD-treated cells

Figure 4: Effects of Akt inhibition on methanolic extract of <i>Dictamnus dasycarpus</i> (MEDD)-induced AGS cell death. (a) Cells were pretreated with the indicated mitogen-activated protein kinases inhibitors (SB203580 (20 μM), SP600125 (20 μM), or PD98059 (50 μM)) for 1 h and then treated with MEDD (350 μg/ml) for 24 h. (b) cells were pretreated with the Akt inhibitor (LY294002 (20 μM)) for 1 h and then treated with MEDD (280 μg/ml) for 24 h. Cell viabilities were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium assay. Results are the means ± standard deviations of two different experiments. *<i>P</i> < 0.05 versus untreated cells; <sup>#</sup><i>P</i> < 0.05 versus MEDD-treated cells; n.s.: Not significant versus MEDD-treated cells