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  Indian J Med Microbiol
 

Figure 3: Effects of methanolic extract of Dictamnus dasycarpus (MEDD) on the protein levels of Bcl-2 and Bcl-xL and on mitochondrial membrane potential levels in AGS cells. (a) Cells were treated with the indicated concentrations of MEDD for 24 h, collected, and incubated with JC-1 (10 μM) for 20 min at 37°C in the dark. They were then washed once with PBS and subjected to deoxyribonucleic acid flow cytometry. (b) Cell lysates obtained from cells grown under the same conditions as (a) They were separated in SDS-polyacrylamide gels and transferred to nitrocellulose membranes, which were probed with the indicated antibodies. Actin was used as an internal control

Figure 3: Effects of methanolic extract of <i>Dictamnus dasycarpus</i> (MEDD) on the protein levels of Bcl-2 and Bcl-xL and on mitochondrial membrane potential levels in AGS cells. (a) Cells were treated with the indicated concentrations of MEDD for 24 h, collected, and incubated with JC-1 (10 μM) for 20 min at 37°C in the dark. They were then washed once with PBS and subjected to deoxyribonucleic acid flow cytometry. (b) Cell lysates obtained from cells grown under the same conditions as (a) They were separated in SDS-polyacrylamide gels and transferred to nitrocellulose membranes, which were probed with the indicated antibodies. Actin was used as an internal control