Close
  Indian J Med Microbiol
 

Figure 2: Activations of caspases and degradation of poly (ADP-ribose) polymerase protein by methanolic extract of Dictamnus dasycarpus (MEDD) in AGS cells. (a) AGS cells were treated with the indicated concentrations of MEDD for 24 h. Membranes were probed with the indicated antibodies. Actin was used as the internal control. (b) After 24 h incubation with the indicated concentrations of MEDD, cells were lysed, and aliquots were assayed for in vitro caspase-3 and -9 activities using DEVD-p-nitroaniline (pNA) and LEHD-pNA as substrates, respectively. Results are the mean ± standard deviations of three different experiments. *P < 0.05 versus untreated control

Figure 2: Activations of caspases and degradation of poly (ADP-ribose) polymerase protein by methanolic extract of <i>Dictamnus dasycarpus</i> (MEDD) in AGS cells. (a) AGS cells were treated with the indicated concentrations of MEDD for 24 h. Membranes were probed with the indicated antibodies. Actin was used as the internal control. (b) After 24 h incubation with the indicated concentrations of MEDD, cells were lysed, and aliquots were assayed for <i>in vitro</i> caspase-3 and -9 activities using DEVD-p-nitroaniline (pNA) and LEHD-pNA as substrates, respectively. Results are the mean ± standard deviations of three different experiments. *<i>P</i> < 0.05 versus untreated control