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  Indian J Med Microbiol
 

Figure 1: Induction of apoptosis by methanolic extract of Dictamnus dasycarpus (MEDD) Turcz in AGS cells. (a) Cells were treated with the indicated concentrations of MEDD for 24 h. Cell viabilities were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium assay. The significances of difference were determined using the Student's t-test (*P < 0.05 vs. untreated cells) (b) to quantify the degree of apoptosis induced by MEDD, cells grown under the same conditions as (a) were evaluated by flow cytometry for sub-G1 DNA contents (a surrogate of apoptotic DNA degradation). Results are the mean ± standard deviations of two different experiments

Figure 1: Induction of apoptosis by methanolic extract of <i>Dictamnus dasycarpus</i> (MEDD) Turcz in AGS cells. (a) Cells were treated with the indicated concentrations of MEDD for 24 h. Cell viabilities were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium assay. The significances of difference were determined using the Student's <i>t</i>-test (*<i>P</i> < 0.05 vs. untreated cells) (b) to quantify the degree of apoptosis induced by MEDD, cells grown under the same conditions as (a) were evaluated by flow cytometry for sub-G1 DNA contents (a surrogate of apoptotic DNA degradation). Results are the mean ± standard deviations of two different experiments