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  Indian J Med Microbiol
 

Figure 5: Inhibitory effect of compound 2 on protein kinase B (AKT) kinase activity. (a and c) Direct inhibition of AKT kinase activity by compound 2 or staurosporin (Stau) was measured using immunoprecipitated or purified AKT (WT or mutant [C310A]). The level of AKT transfected was measured by immunoblotting analysis. (b) After immunoblotting, levels of phospho-or total AKT in whole lysates from lipopolysaccharide (LPS)-activated RAW264.7 cells pretreated with compound 2 (5 and 10 μM) were identified using specific antibodies. (d) Level of nitric oxide was determined by the Griess assay from the culture supernatants of RAW264.7 cells treated with standard compounds (LY294002 [LY] and wortmannin [Wort]), and LPS (1 μg/ml) for 24 h. All of the data are presented as the mean ± standard error of the mean of three different experiments performed with three samples *P < 0.05 and **P < 0.01 compared to control or normal

Figure 5: Inhibitory effect of compound 2 on protein kinase B (AKT) kinase activity. (a and c) Direct inhibition of AKT kinase activity by compound 2 or staurosporin (Stau) was measured using immunoprecipitated or purified AKT (WT or mutant [C310A]). The level of AKT transfected was measured by immunoblotting analysis. (b) After immunoblotting, levels of phospho-or total AKT in whole lysates from lipopolysaccharide (LPS)-activated RAW264.7 cells pretreated with compound 2 (5 and 10 μM) were identified using specific antibodies. (d) Level of nitric oxide was determined by the Griess assay from the culture supernatants of RAW264.7 cells treated with standard compounds (LY294002 [LY] and wortmannin [Wort]), and LPS (1 μg/ml) for 24 h. All of the data are presented as the mean ± standard error of the mean of three different experiments performed with three samples *<i>P</i> < 0.05 and **<i>P</i> < 0.01 compared to control or normal