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  Indian J Med Microbiol
 

Figure 4: Inhibitory effects of compound 2 on the production of nitric oxide (NO) and PGE2, the expression of inflammatory genes, and the viability of RAW264.7 cells. (a: Left panel and right panel) Levels of NO and PGE2 were determined by the Griess assay, ELISA, and EIA from the culture supernatants of RAW264.7 cells treated with compound 2 (0–12.5 μM), standard compounds (L-NAME and indomethacine), and lipopolysaccharide (LPS) (1 μg/ml) for 24 h. (b) The mRNA levels of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α, and interleukin-6 were determined by reverse transcriptase-polymerase chain reaction using RAW264.7 cells treated with compound 2 (0–12.5 μM) and LPS (1 μg/ml) for 24 h. (c) Cell viability of RAW264.7 cells treated with compound 2 (0–12.5 μM) was determined by 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole assay. All data are presented as the mean ± standard error of the mean of three different experiments performed with three samples (*P < 0.05 and **P < 0.01 compared to control or normal group)

Figure 4: Inhibitory effects of compound 2 on the production of nitric oxide (NO) and PGE2, the expression of inflammatory genes, and the viability of RAW264.7 cells. (a: Left panel and right panel) Levels of NO and PGE2 were determined by the Griess assay, ELISA, and EIA from the culture supernatants of RAW264.7 cells treated with compound 2 (0–12.5 μM), standard compounds (L-NAME and indomethacine), and lipopolysaccharide (LPS) (1 μg/ml) for 24 h. (b) The mRNA levels of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α, and interleukin-6 were determined by reverse transcriptase-polymerase chain reaction using RAW264.7 cells treated with compound 2 (0–12.5 μM) and LPS (1 μg/ml) for 24 h. (c) Cell viability of RAW264.7 cells treated with compound 2 (0–12.5 μM) was determined by 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole assay. All data are presented as the mean ± standard error of the mean of three different experiments performed with three samples (*<i>P</i> < 0.05 and **<i>P</i> < 0.01 compared to control or normal group)