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  Indian J Med Microbiol
 

Figure 8: (a) Changes in nuclear morphology observed by diaminophenylindole (DAPI) staining (×20 and × 40). Figures represent the gradual increase of DAPI-fluorescence intensity due to increase of DNA nick with the increase of time-points of Con A treatment in H460 cells. The representative arrows denote the DNA nicks and nuclear deformities (b) acridine orange/ethidium bromide (AO/EB) dual-staining assay (×20). The gradual increase of the EB-florescence intensity was observed in Con A-treated cells, but remarkably observed at 24 h time-point, indicated the fragmentation of DNA (indicated by arrows). (C) DNA fragmentation assay represents the formation of DNA laddering (indicated with arrows) specifically at 18 h and more in 24 h time-point of Con A treatment in respect to UT due to DNA breakage which indicates apoptosis generation at late hours of Con A treatment. Deoxyuridine triphosphate end labelling by TUNEL assay. Figures showed the gradual increase of TUNEL-positive cells with increase of time of Con A treatment against UT and the maximum DNA breakage was observed at 24 h of treatment (39.41%)

Figure 8: (a) Changes in nuclear morphology observed by diaminophenylindole (DAPI) staining (×20 and × 40). Figures represent the gradual increase of DAPI-fluorescence intensity due to increase of DNA nick with the increase of time-points of Con A treatment in H460 cells. The representative arrows denote the DNA nicks and nuclear deformities (b) acridine orange/ethidium bromide (AO/EB) dual-staining assay (×20). The gradual increase of the EB-florescence intensity was observed in Con A-treated cells, but remarkably observed at 24 h time-point, indicated the fragmentation of DNA (indicated by arrows). (C) DNA fragmentation assay represents the formation of DNA laddering (indicated with arrows) specifically at 18 h and more in 24 h time-point of Con A treatment in respect to UT due to DNA breakage which indicates apoptosis generation at late hours of Con A treatment. Deoxyuridine triphosphate end labelling by TUNEL assay. Figures showed the gradual increase of TUNEL-positive cells with increase of time of Con A treatment against UT and the maximum DNA breakage was observed at 24 h of treatment (39.41%)