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  Indian J Med Microbiol
 

Figure 10: (a) Study on apoptosis markers at mRNA level by reverse transcriptase polymerase chain reaction (RT-PCR) and (b) at protein level by enzyme linked immunosorbent assay (ELISA). The results of RT-PCR and ELISA of Bax/Bcl2 revealed significant upregulation of Bax and downregulation of Bcl2 at 12 h of treatment against untreated samples. The results of cytochrome-c expression at mRNA and protein level revealed that at 18 h of treatment cytochrome-c expression was at the maximum as compared to the untreated control. Significant increase of expression of caspse-3 at 24 h interval was observed against UT that triggers apoptosis. Results are expressed as mean ± standard deviation (SD) (N = 6). Significance, *P < 0.05 versus untreated (UT) and †P < 0.001 versus UT. (c) Study on apoptosis markers at protein level by western blot. The results of Bax/Bcl2 revealed significant upregulation of Bax and downregulation of Bcl2 at 12 h of treatment against untreated samples. The results of cytochrome-c expression revealed that at 18 h of treatment cytochrome-c expression was at the maximum as compared to the untreated control. Significant increase of expression of caspse-3 at 24 h interval was observed against UT that stimulates the cleavage of PARP. The western blot analysis of PARP at different hour intervals showed that at 24 h time-point, PARP cleavage occurred by the formation of active 89 kDa and inactive 116 kDa subunits where band-intensity was significantly increased in 89 kDa fragment against untreated controls. Results are expressed as mean ± SD (N = 6). Significance, *P < 0.05 versus untreated (UT) and †P < 0.001 versus UT

Figure 10: (a) Study on apoptosis markers at mRNA level by reverse transcriptase polymerase chain reaction (RT-PCR) and (b) at protein level by enzyme linked immunosorbent assay (ELISA). The results of RT-PCR and ELISA of Bax/Bcl2 revealed significant upregulation of Bax and downregulation of Bcl2 at 12 h of treatment against untreated samples. The results of cytochrome-c expression at mRNA and protein level revealed that at 18 h of treatment cytochrome-c expression was at the maximum as compared to the untreated control. Significant increase of expression of caspse-3 at 24 h interval was observed against UT that triggers apoptosis. Results are expressed as mean ± standard deviation (SD) (<i>N</i> = 6). Significance, *<i>P</i> < 0.05 versus untreated (UT) and †<i>P</i> < 0.001 versus UT. (c) Study on apoptosis markers at protein level by western blot. The results of Bax/Bcl2 revealed significant upregulation of Bax and downregulation of Bcl2 at 12 h of treatment against untreated samples. The results of cytochrome-c expression revealed that at 18 h of treatment cytochrome-c expression was at the maximum as compared to the untreated control. Significant increase of expression of caspse-3 at 24 h interval was observed against UT that stimulates the cleavage of PARP. The western blot analysis of PARP at different hour intervals showed that at 24 h time-point, PARP cleavage occurred by the formation of active 89 kDa and inactive 116 kDa subunits where band-intensity was significantly increased in 89 kDa fragment against untreated controls. Results are expressed as mean ± SD (<i>N</i> = 6). Significance, *<i>P</i> < 0.05 versus untreated (UT) and †<i>P</i> < 0.001 versus UT