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  Indian J Med Microbiol
 

Figure 9: (a) Enzyme linked immunosorbent assay (ELISA) of cell cycle markers. Results of ELISA showed significant downregulation of cyclin D1-CDK2 with p21 and p53 up-regulation in the early phases (2 h, 6 h, 12 h) of Con A treatment (32 µg/ml), specifically at 12 h against untreated samples. But in the late phases of treatment (18 h and 24 h), downregulation of cyclin D1-CDK1 and p21 was found with the significant increased expression of p53 (b) Western blots of cell cycle markers. Results showed significant downregulation of cyclin D1-CDK2 with p21 and p53 up-regulation in the early phases (2 h, 6 h, 12 h) of Con A treatment (32 µg/ml), specifically at 12 h against untreated samples. But in the late phases of treatment (18 h and 24 h), downregulation of cyclin D1-CDK1 and p21 was found with the significant increased expression of p53. Results are expressed as mean ± standard deviation (N = 6). Significance, *P < 0.05 versus untreated (UT) and †P < 0.001 versus UT

Figure 9: (a) Enzyme linked immunosorbent assay (ELISA) of cell cycle markers. Results of ELISA showed significant downregulation of cyclin D1-CDK2 with p21 and p53 up-regulation in the early phases (2 h, 6 h, 12 h) of Con A treatment (32 µg/ml), specifically at 12 h against untreated samples. But in the late phases of treatment (18 h and 24 h), downregulation of cyclin D1-CDK1 and p21 was found with the significant increased expression of p53 (b) Western blots of cell cycle markers. Results showed significant downregulation of cyclin D1-CDK2 with p21 and p53 up-regulation in the early phases (2 h, 6 h, 12 h) of Con A treatment (32 µg/ml), specifically at 12 h against untreated samples. But in the late phases of treatment (18 h and 24 h), downregulation of cyclin D1-CDK1 and p21 was found with the significant increased expression of p53. Results are expressed as mean ± standard deviation (<i>N</i> = 6). Significance, *<i>P</i> < 0.05 versus untreated (UT) and †<i>P</i> < 0.001 versus UT