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  Indian J Med Microbiol
 

Figure 4: Effects of MA128 on the levels of melanogenic proteins, p-c-AMP-dependent protein kinase (PKA), and p-c-AMP-related element binding protein (CREB) in alpha-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 cells. (a) B16F10 cells preincubated with or without 100 and 250 μg/mL MA128 for 12 h were simulated with 1 μM α-MSH for additional 36 h. After harvest of cells, lysates were subjected to western blotting for the detection of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor levels. (b) B16F10 cells were preincubated with or without 100 and 250 μg/mL MA128 for 12 h, and then further stimulated with 1 μM α-MSH for 30 min. Changes in the level of p-PKA and p-CREB were examined by western blotting followed by quantitation with ImageJ. Relative ratios were determined after normalization to α-tubulin expression. Data are expressed as mean ± standard deviation of two independent experiments. *P < 0.05 versus untreated control, #P < 0.05 versus α-MSH stimulation

Figure 4: Effects of MA128 on the levels of melanogenic proteins, p-c-AMP-dependent protein kinase (PKA), and p-c-AMP-related element binding protein (CREB) in alpha-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 cells. (a) B16F10 cells preincubated with or without 100 and 250 μg/mL MA128 for 12 h were simulated with 1 μM α-MSH for additional 36 h. After harvest of cells, lysates were subjected to western blotting for the detection of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor levels. (b) B16F10 cells were preincubated with or without 100 and 250 μg/mL MA128 for 12 h, and then further stimulated with 1 μM α-MSH for 30 min. Changes in the level of p-PKA and p-CREB were examined by western blotting followed by quantitation with ImageJ. Relative ratios were determined after normalization to α-tubulin expression. Data are expressed as mean ± standard deviation of two independent experiments. <i>*P</i> < 0.05 versus untreated control, <i>#P</i> < 0.05 versus α-MSH stimulation