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  Indian J Med Microbiol
 

Figure 2: Effects of MA128 on the expression levels of melanogenic proteins in B16F10 cells. (a) B16F10 cells were treated with 50, 100, and 250 μg/mL MA128 for 48 h. Harvested cells were lysed and then examined for the expression levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor by western blotting. After normalization to α-tubulin, relative ratios were quantitated with ImageJ. (b) The levels of p-mitogen-activated protein kinases, including p-p38, p-extracellular signal-related kinase 1/2, and p-c-Jun-N-terminal kinase were also examined by western blotting. Relative ratios of phosphorylated forms to total levels were determined after normalization to α-tubulin expression. Data are expressed as mean ± standard deviation of two independent experiments. *P < 0.05 versus untreated control

Figure 2: Effects of MA128 on the expression levels of melanogenic proteins in B16F10 cells. (a) B16F10 cells were treated with 50, 100, and 250 μg/mL MA128 for 48 h. Harvested cells were lysed and then examined for the expression levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor by western blotting. After normalization to α-tubulin, relative ratios were quantitated with ImageJ. (b) The levels of p-mitogen-activated protein kinases, including p-p38, p-extracellular signal-related kinase 1/2, and p-c-Jun-N-terminal kinase were also examined by western blotting. Relative ratios of phosphorylated forms to total levels were determined after normalization to α-tubulin expression. Data are expressed as mean ± standard deviation of two independent experiments. <i>*P</i> < 0.05 versus untreated control