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  Indian J Med Microbiol
 

Figure 1: Effects of MA128 on the cell viability and melanogenic activity in B16F10 cells. (a) B16F10 cells and HaCaT cells seeded onto a 96-well culture plate were incubated with indicated concentrations of MA128 for 48 h, and then the cell viability was estimated by 3-4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay. (b) B16F10 cells were incubated with or without 100 and 250 μg/ml MA128 for 48 h, and then observed for the accumulation of melanin under phase contrast microscope. Image magnification, ×200. (c) B16F10 cells (1 × 107) incubated for 48 h with or without 100 and 250 μg/ml MA128 were determined for the melanin content. (d) Cellular tyrosinase activity in B16F10 cells was examined by measuring the L-3,4-Dihydroxyphenylalanine oxidation. Each percentage value was calculated by comparing to that of untreated "control" cells. Data are expressed as the mean ± standard deviation of two independent experiments. *P < 0.05 versus untreated control

Figure 1: Effects of MA128 on the cell viability and melanogenic activity in B16F10 cells. (a) B16F10 cells and HaCaT cells seeded onto a 96-well culture plate were incubated with indicated concentrations of MA128 for 48 h, and then the cell viability was estimated by 3-4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay. (b) B16F10 cells were incubated with or without 100 and 250 μg/ml MA128 for 48 h, and then observed for the accumulation of melanin under phase contrast microscope. Image magnification, ×200. (c) B16F10 cells (1 × 107) incubated for 48 h with or without 100 and 250 μg/ml MA128 were determined for the melanin content. (d) Cellular tyrosinase activity in B16F10 cells was examined by measuring the L-3,4-Dihydroxyphenylalanine oxidation. Each percentage value was calculated by comparing to that of untreated *P < 0.05 versus untreated control">