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  Indian J Med Microbiol
 

Figure 3: CMF induced apoptosis through a caspase-mediated pathway in K562 cells. (a) Evaluation of apoptosis by Annexin V-FITC/PI dual staining assay and flow cytometer analysis after CMF treatment (100, 300, 900 μg/ml) for 48 h. (b) Effects of CMF on the expression of caspase-3 and PARP proteins. Equal amounts of whole cell lysates (50 μg) were subjected to Western blot analysis. The amount of GAPDH was measured as an internal control

Figure 3: CMF induced apoptosis through a caspase-mediated pathway in K562 cells. (a) Evaluation of apoptosis by Annexin V-FITC/PI dual staining assay and flow cytometer analysis after CMF treatment (100, 300, 900 μg/ml) for 48 h. (b) Effects of CMF on the expression of caspase-3 and PARP proteins. Equal amounts of whole cell lysates (50 μg) were subjected to Western blot analysis. The amount of GAPDH was measured as an internal control