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  Indian J Med Microbiol
 

Figure 2: Effects of compounds 5 and 6 on COX-2 and iNOS mRNA expression in HepG2 cell lines. HepG2 cells were pretreated in the absence and presence of compounds 5 and 6 for 1 h before TNF-α treatment (10 ng/mL), then exposed to TNF-α for 6 h. Total mRNAs were prepared from the cell pellets using TRIzol. The relative levels of mRNAs were assessed by RT-PCR

Figure 2: Effects of compounds 5 and 6 on COX-2 and iNOS mRNA expression in HepG2 cell lines. HepG2 cells were pretreated in the absence and presence of compounds 5 and 6 for 1 h before TNF-α treatment (10 ng/mL), then exposed to TNF-α for 6 h. Total mRNAs were prepared from the cell pellets using TRIzol. The relative levels of mRNAs were assessed by RT-PCR