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  Indian J Med Microbiol
 

Figure 3: Phosphatidyl serine surface expression and caspase activation. (a) Annexin V and propidium iodide labeling of B16F10- Nex2 (3 x 105) cells grown for 24 h in 12-well plate and incubated with 12 and 25 μg/ml of HE or RPMI medium for 18 h at 37 °C. Positive annexin V (AV) and propidium iodide cells were detected with an inverted fluorescence microscope at 10X magnification; (b) Activation of caspase-2, 3, 8 and 9 in HE treated melanoma cells. B16F10-Nex2 cells (107) were treated with 50 μg/ml of HE for 24 h, and the HE induced enzymatic activity of caspase-2, 3, 8 and 9 was evaluated by colorimetric assay

Figure 3: Phosphatidyl serine surface expression and caspase activation. (a) Annexin V and propidium iodide labeling of B16F10- Nex2 (3 x 105) cells grown for 24 h in 12-well plate and incubated with 12 and 25 μg/ml of HE or RPMI medium for 18 h at 37 °C. Positive annexin V (AV) and propidium iodide cells were detected with an inverted fluorescence microscope at 10X magnification; (b) Activation of caspase-2, 3, 8 and 9 in HE treated melanoma cells. B16F10-Nex2 cells (107) were treated with 50 μg/ml of HE for 24 h, and the HE induced enzymatic activity of caspase-2, 3, 8 and 9 was evaluated by colorimetric assay