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  Indian J Med Microbiol
 

Figure 2: PCR analysis for hairy roots lines of L. mucronatum. (a) PCR analysis using the rolA and rolB genes specific primers. M: 1 Kb DNA Ladder (Fermantas), 1: Ri plasmid from A. rhizogenes strain 'A13' as a positive control, 2 to 3: Transgenic hairy roots induced on hypocotyl and root ex-plants infected by Agrobacterium, 4: Wild plant root as first negative control, 5: Non-DNA template PCR reaction as second negative control. (b) PCR analysis using virD genes specific primers. M: 1 Kb DNA Ladder (Fermantas), 1: Ri plasmid from A. rhizogenes strain 'A13' as a positive control, 2, 3: Transgenic hairy roots induced on hypocotyl and root explants infected by Agrobacterium, 4: Wild plant root as fi rst negative control, 5: Non-DNA template PCR reaction as second negative control

Figure 2: PCR analysis for hairy roots lines of <i>L. mucronatum</i>. (a) PCR analysis using the <i>rolA</i> and <i>rolB</i> genes specific primers. M: 1 Kb DNA Ladder (Fermantas), 1: Ri plasmid from <i>A. rhizogenes</i> strain 'A13' as
a positive control, 2 to 3: Transgenic hairy roots induced on hypocotyl and root ex-plants infected by Agrobacterium, 4: Wild plant root as first negative control, 5: Non-DNA template PCR reaction as second negative control. (b) PCR analysis using virD genes specific primers. M: 1 Kb DNA Ladder (Fermantas), 1: Ri plasmid from <i>A. rhizogenes</i> strain 'A13' as a positive control, 2, 3: Transgenic hairy roots induced on hypocotyl and root explants infected by Agrobacterium, 4: Wild plant root as fi rst negative control, 5: Non-DNA template PCR reaction as second negative control