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  Indian J Med Microbiol
 

Figure 1: Rat cortical cultures were washed with Dulbecco's modified Eagle's medium and incubated with the methanolic extract of L. japonica for 1 hour. The cultures were then exposed to 100 mM glutamate for 24 hours. After the incubation, the cultures were assessed for the extent of neuronal damage (treatment throughout). The values shown are the mean ± Standard Deviation of three experiments (5 - 6 cultures per experiment). alactate dehydrogenase (LDH) released from control and glutamate-treated cultures was 115.8 ± 3.8 and 212.8 ± 5.9 mU/ml, respectively. Cell viability was calculated as 100 × (LDH released from glutamate-treated - LDH released from glutamate+test compound) / (LDH released from glutamate-treated - LDH released from control), bGlutamate-treated value differs significantly from the untreated control at a level of P < 0.001, cMK 801: dizocilpine maleate, a non-competitive antagonist of the N-methyl D-aspartate (NMDA) receptor, dCNQX: 6-cyano-7-nitroquinoxaline-2, 3-dione, non-NMDA receptor antagonist *P < 0.05, **P < 0.01, ***P < 0.001 vs glutamate-treated cultures (Analysis of Variance [ANOVA] and Tukey)

Figure 1: Rat cortical cultures were washed with Dulbecco's modified Eagle's medium and incubated with the methanolic extract of <i>L. japonica</i> for 1 hour. The cultures were then exposed to 100 mM glutamate for 24 hours. After the incubation, the cultures were assessed for the extent of neuronal damage (treatment throughout). The values shown are the mean ± Standard Deviation of three experiments (5 - 6 cultures per experiment). alactate dehydrogenase (LDH) released from control and glutamate-treated cultures was 115.8 ± 3.8 and 212.8 ± 5.9 mU/ml, respectively. Cell viability was calculated as 100 × (LDH released from glutamate-treated - LDH released from glutamate+test compound) / (LDH released from glutamate-treated - LDH released from control), bGlutamate-treated value differs significantly from the untreated control at a level of <i>P</i> < 0.001, <sup>c</sup>MK 801: dizocilpine maleate, a non-competitive antagonist of the N-methyl D-aspartate (NMDA) receptor, <sup>d</sup>CNQX: 6-cyano-7-nitroquinoxaline-2, 3-dione, non-NMDA receptor antagonist *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001 vs glutamate-treated cultures (Analysis of Variance [ANOVA] and Tukey)