Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 16  |  Issue : 69  |  Page : 329--335

Neuroprotective compounds from the embryo of Nelumbo nucifera seeds


Jin Bae Weon1, Choong Je Ma2 
1 Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, South Korea
2 Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, South Korea

Correspondence Address:
Choong Je Ma
Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Kangwondaehak-gil 1, Chuncheon 24341
South Korea

Background: Previous studies have shown the cognitive effect of the embryo of Nelumbo nucifera on scopolamine-induced memory impairment and neuroprotective effect against glutamate-injured neurotoxicity in HT-22 cells. Objectives: The present study was designed for the purpose of evaluating the neuroprotective activity of compounds isolated from the embryo of N. nucifera seeds on glutamate-induced cell death in HT-22 cells. Materials and Methods: We isolated compound from the embryo of N. nucifera using various chromatograms and confirmed chemical structure by various spectroscopy. The neuroprotective effects of the compounds against glutamate-induced cell death in HT-22 cells were investigated using an (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: We isolated 6 compounds, 1,2,3,4-tetrahydro-7,8-isoquinolinediol (1), 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-6,7-Isoquinoline diol (2), 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl -7-Isoquinolinol(3),1-(3,4,5-trihydroxyphenyl)-ethanone(4), 1-(2,3,5,6-tetrahydroxyphenyl)-ethanone(5),3-(prop-1-enyl) benzene-1,2,4,5-tetrol (6) from the embryo of N. nucifera . Among these compounds, 1,2,3,4-tetrahydro-7,8-isoquinolinediol and 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl)methyl]-2-methyl-6,7-Isoquino linediol significantly decreased glutamate-induced cell death in HT-22 cells. In addition, these compounds exacerbated the reactive oxygen species level and intracellular Ca2+ accumulation. Conclusion: The neuroprotective efficacy of 1,2,3,4-tetrahydro-7,8-isoquinolinediol and 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-6,7-Isoquinolinediolmay be related to their antioxidative effect.


How to cite this article:
Weon JB, Ma CJ. Neuroprotective compounds from the embryo of Nelumbo nucifera seeds.Phcog Mag 2020;16:329-335


How to cite this URL:
Weon JB, Ma CJ. Neuroprotective compounds from the embryo of Nelumbo nucifera seeds. Phcog Mag [serial online] 2020 [cited 2020 Aug 13 ];16:329-335
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=69;spage=329;epage=335;aulast=Weon;type=0