Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 15  |  Issue : 63  |  Page : 423--432

Angiopteris helferiana, a fern with great potential medicinal value: Antiadipogenic, anti-inflammatory, and anti-diabetic activity


Ramakanta Lamichhane1, Prakash Raj Pandeya1, Kyung-Hee Lee1, Se-Gun Kim2, Dhan Raj Kandel3, Hyun-Ju Jung1 
1 Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Republic of Korea
2 Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea
3 Department of Plant Resources, National Herbarium and Plant Laboratories, Lalitpur, Nepal

Correspondence Address:
Hyun-Ju Jung
Department of Oriental Pharmacy, Wonkwang University, Iksan 54538
Republic of Korea

Background: Angiopteris helferiana is used as a traditional medicine in some parts of Nepal and South-Asian countries. It is also used in traditional Chinese medicine system. Objective: In this study, we evaluated its anti-oxidant, anti-inflammatory, anti-obesity, and antidiabetic activity in cell and animal mode. Materials and Methods: The rhizome of A. helferiana was extracted with methanol and fractionated to give dichloromethane, ethyl acetate (EA), butanol (BuOH), and water fractions. The residue was again re-extracted with water to give water extract. The anti-oxidant property was measured using 1, 1-diphenyl-2-picryl-hydrazyl assay. The extract and fractions were evaluated for the α-glycosidase inhibition activity. The antiadipogenic and anti-inflammatory activity was studied in 3T3-L1–L1 and RAW 264.7 cells, respectively. The inhibition of expression of adipogenic markers peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT enhancer binding protein alpha (C/EBPα) was studied in 3T3-L1 cells. The anti-obesity and antidiabetic study was done in high-fat diet mice model. Results: The EA, BuOH fraction, and water extract showed good anti-oxidant and α-glycosidase inhibition activity. EA fraction showed good anti-inflammatory activity. EA and BuOH fraction showed good antiadipogenic activity, reducing significantly, the lipid and adipogenic markers (PPARγ, C/EBPα) in 3T3-L1 cells. The BuOH fraction at 300 mg/kg/day dose showed good anti-obesity and antidiabetic activity in the in-vivo study after the evaluation of body weight, blood lipid, blood glucose, and lipid accumulation in adipose tissue and liver. Conclusion: Overall, A. helferiana is a good source of anti-oxidative, anti-inflammatory, anti-obesity, and antidiabetic component.


How to cite this article:
Lamichhane R, Pandeya PR, Lee KH, Kim SG, Kandel DR, Jung HJ. Angiopteris helferiana, a fern with great potential medicinal value: Antiadipogenic, anti-inflammatory, and anti-diabetic activity.Phcog Mag 2019;15:423-432


How to cite this URL:
Lamichhane R, Pandeya PR, Lee KH, Kim SG, Kandel DR, Jung HJ. Angiopteris helferiana, a fern with great potential medicinal value: Antiadipogenic, anti-inflammatory, and anti-diabetic activity. Phcog Mag [serial online] 2019 [cited 2020 Jun 5 ];15:423-432
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2019;volume=15;issue=63;spage=423;epage=432;aulast=Lamichhane;type=0