Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 14  |  Issue : 55  |  Page : 154--160

Intercomparative investigation of the total phenols, total flavonoids, In vitro and In vivo antioxidant activities of capparis Cartilaginea (Decne.) maire and weiller and Capparis Ovata Desf. from Jordan


Mahmoud A Al-Qudah1, Safwan M Obeidat1, Riyadh Muhaidat2, Bahaa Al-Trad2, Hala I Al-Jaber3, Jamil N Lahham2 
1 Department of Chemistry, Faculty of Science, Yarmouk University, Irbid, Jordan
2 Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan
3 Department of Physics and Basic Sciences, Faculty of Engineering Technology, Al-Balqa Applied University, Amman, Jordan

Correspondence Address:
Mahmoud A Al-Qudah
Department of Chemistry, Faculty of Science, Yarmouk University, P.O. Box 566, Irbid 21163
Jordan

Background: Plant-derived antioxidants have been products of choice in therapeutic formulations for their potent free radical scavenging activity and mitigating and curing illnesses and diseases associated with oxidative stress. Capparis L. includes species with unprecedented nutritional and medicinal values, and hence offers a diverse pool of phytochemicals implicated in antioxidant activity. Objective: The current study was designed to assess the chemical variation, total phenols content (TPC), total flavonoids content (TFC), in vitro and in vivo antioxidant activities of different extracts obtained from Capparis cartilaginea and Capparis ovata from Jordan. Materials and Methods: The ethanolic extract from each species was partitioned in different solvents and the TPC, TFC, and antioxidant activity for each extract was examined. The in vitro antioxidant activities were tested by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-Azino–bis (3-ethylbenzoline-6-sulfonic acid diammonium salt (ABTS) radical scavenging methods in addition to the metal ion chelating effect (MCE) and hydroxyl radical assay methods. The butanol extract was investigated for its in vivo effect on the activities of serum superoxide dismutase (SOD) and glutathione peroxidase (GPX) in mice (100 mg/kg, intraperitoneally for 12 days). Results: The butanol fraction of both plants had a dose-dependent in vitro antioxidant activity. The treatment of mice with the butanol extract of both species for 12 days significantly increased the activities of serum SOD and GPX. Principal component analysis indicated that DPPH, TFC, hydroxyl radical had major variability in the antioxidant activities of the two investigated Capparis species. Results of this study underscores enrichment of the two studied Capparis species with phenolics and flavonoids that could account for much of their antioxidant activities. Abbreviations used: C. cartilaginea: Capparis cartilaginea ; C. ovata: Capparis ovata; TPC: Total phenols content; TFC: Total flavonoids content; DPPH: 2,2-diphenyl-1-picrylhydrazyl; ABTS: 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid; MC: Metal Chelating effect; Hydroxy: Hydroxyl radical assay; SOD: Serum superoxide dismutase; GPX: Glutathione Peroxidase ;PC: Principal Component.


How to cite this article:
Al-Qudah MA, Obeidat SM, Muhaidat R, Al-Trad B, Al-Jaber HI, Lahham JN. Intercomparative investigation of the total phenols, total flavonoids, In vitro and In vivo antioxidant activities of capparis Cartilaginea (Decne.) maire and weiller and Capparis Ovata Desf. from Jordan.Phcog Mag 2018;14:154-160


How to cite this URL:
Al-Qudah MA, Obeidat SM, Muhaidat R, Al-Trad B, Al-Jaber HI, Lahham JN. Intercomparative investigation of the total phenols, total flavonoids, In vitro and In vivo antioxidant activities of capparis Cartilaginea (Decne.) maire and weiller and Capparis Ovata Desf. from Jordan. Phcog Mag [serial online] 2018 [cited 2020 Jun 1 ];14:154-160
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=55;spage=154;epage=160;aulast=Al-Qudah;type=0