Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 14  |  Issue : 54  |  Page : 149--154

In vitro induction and generation of tetraploid plants of Sophora tonkinensis Gapnep


Kun Hua Wei1, Jian Ping Xu2, Lin Xuan Li1, Jin Yuan Cai3, Jian Hua Miao1, Min Hui Li4 
1 Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, Guangxi, People's Republic of China
2 Inner Mongolia Research Center of Characteristic Medicinal Plants Cultivation and Protection Engineering Technology , Baotou Medical College, Baotou 014060, Inner Mongolia, People's Republic of China
3 Department of Food and Chemical Engineering, Lushan College of Guangxi University of Science and Technology, Liuzhou 545616, Guangxi, People's Republic of China
4 Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, Guangxi; Inner Mongolia Research Center of Characteristic Medicinal Plants Cultivation and Protection Engineering Technology , Baotou Medical College, Baotou 014060, Inner Mongolia, People's Republic of China

Correspondence Address:
Kun Hua Wei
Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, Guangxi
People's Republic of China
Min Hui Li
Inner Mongolia Research Center of Characteristic Medicinal Plants Cultivation and Protection Engineering Technology, Baotou Medical College, Baotou 014060, Inner Mongolia
People's Republic of China

Background: Sophora tonkinensis Gapnep. is an important medical plant in China. Early researches of S. tonkinensis were focused on rapid propagation and quality analysis of in vitro tissue culture plantlet, and still no research focuses on the plant breeding of and there were no excellent varieties for artificial cultivation of S. tonkinensis. Objective: To set up a method to generate and select the best varieties of S. tonkinensis by polyploid breeding after induction by colchicine treatment. Materials and Methods: The adventitious buds were submerged in different concentrations of aqueous colchicine solution for different lengths of time to induce polyploidy in the plants, and the induced buds were identified by root-tip chromosome determination and leaf characteristics comparison. The contents of matrine and oxymatrine of radix ex rhizoma in 13 selected tetraploid lines were collected after 90 days in vitro rooting culture and were evaluated to provide evidence of good qualities of tetraploid S. tonkinensis. Results: The results showed that the highest percentage of tetraploid induction was 23.33% and occurred in the 0.2% (w/v) colchicine treatment for 30 h. Fifty lines of tetraploid plants were obtained and 12 of the 13 selected tetraploid lines exhibited higher productivity of total contents of matrine and oxymatrine when compared to controls. Conclusion: The data demonstrate that polyploidy induction can be beneficial for improving the medicinal value of S. tonkinensis. Abbreviations used: MS medium: Murashige and Skoog medium; BAP: 6-benzylaminopurine; NAA: A-naphthaleneacetic acid; IAA: Indole-3-acetic acid; KT: Kinetin; IBA: Indole-3-butyric acid; ABT: Rooting power.


How to cite this article:
Wei KH, Xu JP, Li LX, Cai JY, Miao JH, Li MH. In vitro induction and generation of tetraploid plants of Sophora tonkinensis Gapnep.Phcog Mag 2018;14:149-154


How to cite this URL:
Wei KH, Xu JP, Li LX, Cai JY, Miao JH, Li MH. In vitro induction and generation of tetraploid plants of Sophora tonkinensis Gapnep. Phcog Mag [serial online] 2018 [cited 2019 Dec 9 ];14:149-154
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=54;spage=149;epage=154;aulast=Wei;type=0