Year : 2017 | Volume
: 13 | Issue : 50 | Page : 334--338
Role of Adiantum philippense L. on glucose uptake in isolated pancreatic cells and inhibition of adipocyte differentiation in 3T3-L1 cell line
Tania Paul1, Kishori G Apte1, Pradeep B Parab1, Biswadeep Das2
1 APT Research Foundation, Pune, Maharashtra, India
2 APT Testing and Research Pvt. Ltd., Pune, Maharashtra, India
Background: Adiantum philippense (AP) is a pteridophyte that shows antihyperglycemic activity in vivo diabetic model, but the mechanism of action is unknown. Objective: AP was found to play a pivotal role in minimizing the high blood glucose in alloxan-induced diabetic rats. Simultaneously, it was observed that it could maintain the normal lipid profile even in diabetic condition. To investigate its insulin-like activity along with its inhibitory role on adipocyte differentiation became the objective of our present study. Materials and Methods: Glucose uptake potential of this fern was done in isolated pancreatic islets and inhibition of adipocyte differentiation was assessed in 3T3-L1 cell line. Before this, the cytotoxic concentration was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on L929 cell line. To determine its role in lipid metabolism, the oil droplets produced in adipocytes were stained with Oil 'O' red staining, and triglyceride levels of various drug treatments were measured spectrophotometrically. Results: This fern extract was found to be actively utilizing glucose in the glucose uptake assay. Moreover, it was also involved in inhibiting differentiation of pro-adipocyte to adipocyte in the 3T3-L1 cell lines. The percentage inhibition as obtained from the absorbance showed that the ethanolic extract at the concentration of 200 μg/ml showed 32.48% inhibition. Conclusion: All the above-mentioned parameters when appraised indicated that this fern could be used as an alternative medicine in managing diabetes associated with obesity.
Abbreviations used: AP: Adiantum phillipense; MTT: (3-(4,5- Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide); BSA: Bovine serum albumin; FCS: Fetal calf serum; DMEM: Dulbecco's minimum essential media; RPMI: Roswell park memorial institute medium; DTZ: Dithizone; TG: Triglyceride; PPARγ: Peroxisome proliferator-activated receptor gamma; IBMX: 3-isobutyl-1-methylxanthine; nm: Nanometer; GI: Growth Inhibition; ELISA: Enzyme linked immunosorbent assay.
APT Research Foundation, S. No. 36/1/1, M.N. 199, Vadgaon Khurd, Sinhagad Road, Pune - 411 041, Maharashtra
|How to cite this article:|
Paul T, Apte KG, Parab PB, Das B. Role of Adiantum philippense L. on glucose uptake in isolated pancreatic cells and inhibition of adipocyte differentiation in 3T3-L1 cell line.Phcog Mag 2017;13:334-338
|How to cite this URL:|
Paul T, Apte KG, Parab PB, Das B. Role of Adiantum philippense L. on glucose uptake in isolated pancreatic cells and inhibition of adipocyte differentiation in 3T3-L1 cell line. Phcog Mag [serial online] 2017 [cited 2019 Nov 15 ];13:334-338
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=50;spage=334;epage=338;aulast=Paul;type=0