Year : 2017 | Volume
: 13 | Issue : 50 | Page : 222--225
Flavonoids isolated from the flowers of Limonium bicolor and their in vitro antitumor evaluation
Jian Chen1, Jiehui Teng2, Li Ma2, Haiying Tong1, Bingru Ren1, Linshan Wang2, Weilin Li2
1 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, China
2 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China
Background: Limonium bicolor, a halophytic species, can grow in saline or saline-alkali soil, is well known as a traditional Chinese medicine. Recently it attracted much attention for its treatment for cancer. Objective: The present study was performed to evaluate this species from the phytochemical standpoint and the possible relationship between the antitumor activity and its natural products. Materials and methods: The chemical constituents from the flowers of L. bicolor were investigated through bioassay-guided fractionation and isolation. All the individual compounds were characterized by spectroscopic analysis and their potential antitumor activity was tested against three different human tumor cell lines by MTT assays. Results: The EtOAc extract was proven as the most potent fraction and further fractionation led to the isolation of 15 natural flavonoids, which were characterized as luteolin (1), acacetin (2), quercetin (3), isorhamnetin (4), kaempferol (5), eriodictyol (6), kaempferol-3-O-α-L-rhamnoside (7), kaempferol-3-O-β-D-glucoside (8), quercetin-3-O-α-L-rhamnoside (9), quercetin-3-O-β-D-glucoside (10), quercetin-3-O-β-D-galactoside (11), myricetin-3-O-α-L-rhamnoside (12), kaempferol-3-O-(6″-O-galloyl)-β-D-glucoside (13), hesperidin (14) and rutin (15). The biotesting results demonstrated that both compounds 1 and 3 showed good cytotoxicity against human colon cancer cells (LOVO). Compound 5 exhibited relative greater growth inhibition against both human breast cancer cells (MCF-7) and osteosarcoma cell lines (U2-OS) at the concentration of 100 μg/mL. Conclusion: On the basis of these findings, the flavonoids were deduced to be potentially responsible for the antitumor activity of L. bicolor. The preliminary structure–activity relationship analysis suggests that the 3-O-glycosylation moiety in natural flavonoids was not essential for the antiproliferative activity on LOVO and U2-OS cells.
Abbreviation used: MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, EtOAc: Ethyl acetate; LOVO: human colon cancer; MCF-7: human breast, cancer; U2-OS: human osteosarcoma; 5-FU: 5-Fluorouracil; DMSO: dimethyl sulfoxide, NMR: nuclear magnetic resonance; HR-ESI-MS: high resolution electrospray ionization mass chromatography, HPLC: high performance liquid chromatography, EtOH: ethanol; n-BuOH: n-butanol; CC: column chromatography, TLC: thin layer chromatography; PBS: phosphate-buffered saline.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing
|How to cite this article:|
Chen J, Teng J, Ma L, Tong H, Ren B, Wang L, Li W. Flavonoids isolated from the flowers of Limonium bicolor and their in vitro antitumor evaluation.Phcog Mag 2017;13:222-225
|How to cite this URL:|
Chen J, Teng J, Ma L, Tong H, Ren B, Wang L, Li W. Flavonoids isolated from the flowers of Limonium bicolor and their in vitro antitumor evaluation. Phcog Mag [serial online] 2017 [cited 2020 Jul 11 ];13:222-225
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=50;spage=222;epage=225;aulast=Chen;type=0