Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 50  |  Page : 216--221

Anti-inflammatory effects of KOTMIN13: A mixed herbal medicine in LPS-stimulated RAW 264.7 cells and mouse edema models


Eujin Lee1, Sun-Gun Kim1, Na-Young Park1, Hyo-Hyun Park1, Kyu-Tae Jeong1, Jongkeun Choi2, In-Hae Lee2, Hwadong Lee1, Eunkyung Lee1 
1 Research and Development Division, National Development Institute of Korean Medicine, Gyeongsan, Republic of Korea
2 Department of Cosmetic Science, Chungwoon University, Chungnam, Republic of Korea

Correspondence Address:
Dr. Eunkyung Lee
Research and Development Division, National Development Institute of Korean Medicine, Gyeongsan
Republic of Korea

Background: A Korean herbal medicine, KOTMIN13, composed of Inula japonica Thunberg, Trichosanthes kirilowii Maximowicz var. japonica kitamura, Peucedanum praeruptorum Dunn, and Allium macrostemon Bge, has been used for anti-allergic and anti-asthmatic treatment in oriental clinics, but its activity has not been investigated. Materials and Methods: To evaluate the anti-inflammatory activity of KOTMIN13 for in vitro study, LPS-stimulated RAW 264.7 cells were used to induce the production and expression of inflammatory mediators and its mechanisms. 12-O-Tetradecanoylphorobol-13 aceate (TPA)-induced ear edema and carrageenan-induced paw edema models were also used to evaluate the effect of KOTMIN13 on acute inflammation in vivo. Results: KOTMIN13 reduced the release of inflammatory mediators [nitric oxide, prostaglandin E2, interleukin (IL)-1β, and IL-6] and the protein expression of inducible nitric oxide synthase and cyclooxygenase-2 in LPS-stimulated RAW 264.7 cells. Mechanism studies showed the attenuation of LPS-induced NF-κB activation by KOTMIN13 via IκBα degradation abrogation and a subsequent decrease in nuclear p65 levels. Activation of mitogen-activated protein kinases (ERK, JNK, and p38) was also suppressed. Furthermore, KOTMIN13 ameliorated the development of TPA-induced ear edema and carrageenan-induced paw edema in acute inflammatory edema mouse models. Conclusion: Our study demonstrates that KOTMIN13 inhibits inflammatory mediators through the inhibitions of NF-κB and MAPK activities in LPS-induced RAW 264.7 cells, as well as acute inflammation in edema models, indicating that KOTMIN13 is an effective suppressor for anti-inflammatory activities. Abbreviations used: NO: nitric oxide; PGE2: prostaglandin E2; iNOS: inducible NO synthase; COX-2: cyclooxygenase-2; TNF-α: tumor necrosis factor-α; IL: interleukin; NF-κB: nuclear factor kappaB; MAPK: mitogen-activated protein kinases; ERK: extracellular signal regulated kinase; JNK: c-jun N terminal kinase; TPA: 12-O-tetradecanoylphorbol-13-acetate


How to cite this article:
Lee E, Kim SG, Park NY, Park HH, Jeong KT, Choi J, Lee IH, Lee H, Lee E. Anti-inflammatory effects of KOTMIN13: A mixed herbal medicine in LPS-stimulated RAW 264.7 cells and mouse edema models.Phcog Mag 2017;13:216-221


How to cite this URL:
Lee E, Kim SG, Park NY, Park HH, Jeong KT, Choi J, Lee IH, Lee H, Lee E. Anti-inflammatory effects of KOTMIN13: A mixed herbal medicine in LPS-stimulated RAW 264.7 cells and mouse edema models. Phcog Mag [serial online] 2017 [cited 2019 Dec 6 ];13:216-221
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=50;spage=216;epage=221;aulast=Lee;type=0