Year : 2017 | Volume
: 13 | Issue : 50 | Page : 170--173
Isolation of abscisic acid from Korean acacia honey with anti-Helicobacter pylori activity
SeGun Kim1, InPyo Hong1, SoonOk Woo1, HyeRi Jang1, SokCheon Pak2, SangMi Han1
1 Department of Agricultural Biology, National Institute of Agricultural Science, Wanju, Korea
2 School of Biomedical Sciences, Charles Sturt University, Bathurst, NSW, Australia
Background: Helicobacter pylori (H. pylori) is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide. Objective: This study is aimed at evaluating the anti-H. pylori activity of Korean acacia honey and isolating the related active components using organic solvents. Material and Methods: The crude acacia honey was extracted with n-hexane, dichloromethane, ethyl acetate (EtOAc), and n-butanol. The EtOAc extract was subjected to octadecyl-silica chromatography. The extracts and fractions were then examined for anti-H. pylori activity using the agar well diffusion method. The antimicrobial activity of abscisic acid against H. pylori was investigated by determining the minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and by performing a time-kill assay. Results: Abscisic acid related to the botanical origins of acacia honey from Korea has been analyzed using ultra-performance liquid chromatography. The MICs and MBCs of abscisic acid were 2.7 ± 1.3 and 6.9 ± 1.9 μg/mL, respectively. The bactericidal activity of abscisic acid (at 10.8 μg/mL corresponding to 4 × MIC) killed the organism within 36–72 h. These results suggest that abscisic acid isolated from Korean acacia honey has antibacterial activity against H. pylori. Conclusion: Abscisic acid isolated from Korean acacia honey can be therapeutic and may be further exploited as a potential lead candidate for the development of treatments for H. pylori-induced infections.
Abbreviations used: MeOH: Methanol; EtOAc: Ethyl acetate; TSB: Trypticase soy broth; MIC: Minimum inhibitory concentration; MBC: Minimum bactericidal concentration; CFU: Colony-forming units; UPLC: Ultra-performance liquid chromatography; DAD: Diode array detector; UV: Ultraviolet; ODS: Octadecyl-silica; MS: Mass spectrometry; SE: Standard error.
Senior Researcher, Bee Products Application Lab. Agricultural Biology Department, National Institute of Agricultural Science, Rural Development Administration, Wanju
|How to cite this article:|
Kim S, Hong I, Woo S, Jang H, Pak S, Han S. Isolation of abscisic acid from Korean acacia honey with anti-Helicobacter pylori activity.Phcog Mag 2017;13:170-173
|How to cite this URL:|
Kim S, Hong I, Woo S, Jang H, Pak S, Han S. Isolation of abscisic acid from Korean acacia honey with anti-Helicobacter pylori activity. Phcog Mag [serial online] 2017 [cited 2019 Dec 13 ];13:170-173
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=50;spage=170;epage=173;aulast=Kim;type=0