Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 49  |  Page : 142--147

Preparation of ginseng extract with enhanced levels of ginsenosides Rg1 and Rb1 using high hydrostatic pressure and polysaccharide hydrolases


Sasikumar Arunachalam Palaniyandi1, Joo-Won Suh2, Seung Hwan Yang3 
1 Interdisciplinary Program of Biomodulation, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do; Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea
2 Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do; Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea
3 Department of Biotechnology, Chonnam National University, Daehak-ro, Yeosu-si, Jeollanam-do, Korea

Correspondence Address:
Prof. Joo-Won Suh
Division of Bioscience and Bioinformatics & Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggido
Korea
Prof. Seung Hwan Yang
Department of Biotechnology, Chonnam National University, Daehak-ro, Yeosu-si, Jeollanam-do
Korea

Background: Ginsenosides are the principal components responsible for the pharmacological activities of ginseng. Ginsenosides Rg1 and Rb1 are the major compounds recognized as marker substances for quality control of ginseng-based products. These major compounds can be transformed to several pharmacologically active minor ginsenosides by chemical, microbial, and enzymatic means. Materials and Methods: In the present study, a combination of polysaccharide hydrolases and high hydrostatic pressure (HHP) were used to extract ginseng saponins enriched with ginsenosides Rg1 and Rb1. Temperature, pH, time, ginseng-to-water ratio, and pressure were optimized to obtain the maximum amount of Rg1 and Rb1 in the resulting extract using commercial polysaccharide hydrolases. Results: This study showed that treatment with a combination of cellulase, amylase, and pectinase at 100 MPa pressure, pH 4.8, and 45°C for 12 h resulted in higher Rg1 and Rb1 levels in the extract. Conclusion: This study describes a cheap and ecofriendly method for preparing ginseng extract enriched with Rg1 and Rb1. Abbreviations used: ATCC: American Type Culture Collection, Mpa: Mega Pascal


How to cite this article:
Palaniyandi SA, Suh JW, Yang SH. Preparation of ginseng extract with enhanced levels of ginsenosides Rg1 and Rb1 using high hydrostatic pressure and polysaccharide hydrolases.Phcog Mag 2017;13:142-147


How to cite this URL:
Palaniyandi SA, Suh JW, Yang SH. Preparation of ginseng extract with enhanced levels of ginsenosides Rg1 and Rb1 using high hydrostatic pressure and polysaccharide hydrolases. Phcog Mag [serial online] 2017 [cited 2019 Oct 16 ];13:142-147
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=49;spage=142;epage=147;aulast=Palaniyandi;type=0