Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 47  |  Page : 211--218

Expression of cytochrome P450s in the liver of rats administered with Socheongryong-tang, a traditional herbal formula


Seong Eun Jin1, Hyekyung Ha1, Chang-Seob Seo1, Hyeun-Kyoo Shin1, Soo-Jin Jeong2 
1 K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea
2 KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea

Correspondence Address:
Soo-Jin Jeong
KM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811
Republic of Korea

Objective: The purpose of this study was to investigate the potential influences of Socheongryong-tang(SCRT) on the messenger ribonucleic acid (mRNA) and protein expression of cytochrome P450 (CYP450) in vivo. Materials and Methods: SCRT was orally administered to either male or female Sprague-Dawley rats once daily at doses of 0, 1000, 2000, or 5000 mg/kg/day for 13 weeks. The mRNA expression of CYP450s (CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1) in liver tissues was measured by reverse transcription polymerase chain reaction. And then, the protein expression of CYP1A1 and CYP2B1/2 in liver tissues was analyzed by the Western blot. Results: We found no significant influence in the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1 after repeated administration of SCRT for 13 weeks. By contrast, the mRNA and protein expression of hepatic CYP1A1 was increased by repeated SCRT treatment in male rats, but not in female rats. The mRNA and protein expression of hepatic CYP2B1/2 in both genders was increased by administration of SCRT. Conclusion: A caution is needed when SCRT is co-administered with substrates of CYP2B1/2 for clinical usage. In case of male, an attention is also required when SCRT and drugs metabolized by CYP1A1 are taken together. Our findings provide information regarding the safety and effectiveness of SCRT when combined with conventional drugs. SUMMARY
  • Oral administration of Socheongryong-tang for 13 weeks did not affect the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1
  • In male rats, oral administration of Socheongryong-tang for 13 weeks induced the mRNA and protein expression of hepatic CYP1A1 and CYP2B1/2
  • In female rats, oral administration of Socheongryong-tang for 13 weeks induced the mRNA and protein expression of hepatic CYP2B1/2.
Abbreviations used: SCRT: Socheongryong-tang, CYP450: Cytochrome P450, HPLC: High performance liquid chromatography, RT-PCR: Reverse transcription polymerase chain reaction. Soo-Jin Jeong



How to cite this article:
Jin SE, Ha H, Seo CS, Shin HK, Jeong SJ. Expression of cytochrome P450s in the liver of rats administered with Socheongryong-tang, a traditional herbal formula.Phcog Mag 2016;12:211-218


How to cite this URL:
Jin SE, Ha H, Seo CS, Shin HK, Jeong SJ. Expression of cytochrome P450s in the liver of rats administered with Socheongryong-tang, a traditional herbal formula. Phcog Mag [serial online] 2016 [cited 2019 Dec 15 ];12:211-218
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=47;spage=211;epage=218;aulast=Jin;type=0