Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 47  |  Page : 193--197

Antioxidant activity of water-soluble polysaccharides from Brasenia schreberi


Huiwen Xiao, Xueru Cai, Yijun Fan, Aoxue Luo 
 Department of Landscape Plants, Sichuan Agriculture University, 611 830 Sichuan, China

Correspondence Address:
Aoxue Luo
Department of Landscape Plants, Sichuan Agriculture University, 611830 Sichuan
China

Objective: In order to investigate the antioxidant activities of polysaccharides (BPL-1 and BPL-2), one of the most important functional constituents in Brasenia schreberi was isolated from the external mucilage of B. schreberi (BPL-1) and the plant in vivo (BPL-2). This paper examines the relationship between the content of sulfuric radicals and uronic acid in BPL and the antioxidant activity of BPL. Materials and Methods: The free radicals, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and 1,1-diphnyl-2-picrylhydrazyl (DPPH-), were used to determine the antioxidant activity of BPL. The Fourier-transform infrared spectroscopy of BPL-1 and BPL-2 revealed typical characteristics of polysaccharides. Results: The two sample types had different contents. This was proved by their different adsorption peak intensities. The IC50 values of BPL-1 (31.189 mg/ml) and BPL-2 (1.863 mg/ml) showed significant DPPH radical scavenging activity. Based on the quantification of ABTS radical scavenging, the IC50 value of BPL-1 (5.460 mg/ml) was higher than that of BPL-2 (0.239 mg/ml). Therefore, in terms of the reducing power, the IC50 value of BPL-1 was too high to determine, and the IC50 value of BPL-2 was found to be 50.557 mg/ml. Hence, the antioxidant activity and total reducing power were high, and they were greater in BPL-2 than in BPL-1. In addition, BPL-2 was found to have more sulfuric radicals and uronic acid than BPL-1. Conclusion: The contents of sulfuric radicals and uronic acid are significantly correlated to the antioxidant activity and reducing power of BPL; the more sulfuric radicals and uronic acid, the more antioxidant activity and reducing power BPL has. SUMMARY
  • The water-soluble crude polysaccharides obtained from the external mucilage and the Brasenia schreberi plant in vivo were confirmed to have high contents of sulfuric radicals and uronic acid
  • Both BPL-1 and BPL-2 exhibited antioxidative activity and reducing power, and their antioxidative activity gradually improved with increasing concentrations
  • The content of sulfuric radicals and uronic acid in BPL-1 and BPL-2 might explain their high antioxidant activity.
Abbreviations used: BPL-1:Polysaccharide were isolated from the external mucilage of B. Schreberi; BPL-2: Polysaccharide were isolated from the plant in vivo of B. schreberi; BPL:Polysaccharide were isolated from B. Schreberi.



How to cite this article:
Xiao H, Cai X, Fan Y, Luo A. Antioxidant activity of water-soluble polysaccharides from Brasenia schreberi.Phcog Mag 2016;12:193-197


How to cite this URL:
Xiao H, Cai X, Fan Y, Luo A. Antioxidant activity of water-soluble polysaccharides from Brasenia schreberi. Phcog Mag [serial online] 2016 [cited 2019 Aug 25 ];12:193-197
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=47;spage=193;epage=197;aulast=Xiao;type=0