Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 46  |  Page : 303--306

Storing of extracts in polypropylene microcentrifuge tubes yields contaminant peak during ultra-flow liquid chromatographic analysis


Parthraj R Kshirsagar1, Harsha Hegde2, Sandeep R Pai1 
1 Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Belagavi, Karnataka, India
2 Plant Biotechnology and Tissue Culture Division; Traditional and Herbal Medicine Division, Regional Medical Research Centre, Indian Council of Medical Research, Belagavi, Karnataka, India

Correspondence Address:
Sandeep R Pai
Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Belagavi - 590 010, Karnataka
India

Background and Aim: This study was designed to understand the effect of storage in polypropylene microcentrifuge tubes and glass vials during ultra-flow liquid chromatographic (UFLC) analysis. Materials and Methods: One ml of methanol was placed in polypropylene microcentrifuge tubes (PP material, Autoclavable) and glass vials (Borosilicate) separately for 1, 2, 4, 8, 10, 20, 40, and 80 days intervals stored at −4°C. Results: Contaminant peak was detected in methanol stored in polypropylene microcentrifuge tubes using UFLC analysis. The contaminant peak detected was prominent, sharp detectable at 9.176 ± 0.138 min on a Waters 250–4.6 mm, 4 μ, Nova-Pak C18 column with mobile phase consisting of methanol:water (70:30). Conclusion: It was evident from the study that long-term storage of biological samples prepared using methanol in polypropylene microcentrifuge tubes produce contaminant peak. Further, this may mislead in future reporting an unnatural compound by researchers.


How to cite this article:
Kshirsagar PR, Hegde H, Pai SR. Storing of extracts in polypropylene microcentrifuge tubes yields contaminant peak during ultra-flow liquid chromatographic analysis.Phcog Mag 2016;12:303-306


How to cite this URL:
Kshirsagar PR, Hegde H, Pai SR. Storing of extracts in polypropylene microcentrifuge tubes yields contaminant peak during ultra-flow liquid chromatographic analysis. Phcog Mag [serial online] 2016 [cited 2019 Dec 10 ];12:303-306
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=46;spage=303;epage=306;aulast=Kshirsagar;type=0