Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 46  |  Page : 128--133

In vitro and In vivo antioxidant activity of flavonoid extracted from mulberry fruit (Morus alba L.)


Sivakumar Thasma Raman, Ajay Krishna Palani Gounder Ganeshan, Cheng Chen, Chao Jin, Shao-Hui Li, Hui-Juan Chen, Zhongzheng Gui 
 School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, P.R. China

Correspondence Address:
Zhongzheng Gui
School of Biotechnology, Jiangsu University of Science and Technology, Sibaidu, Zhenjiang 212018, Jiangsu Province
P.R. China

Background: Many plants possess antioxidants that exhibit additive or synergistic activities. Objective: In this study, an ethanol-extracted flavonoid extracted from mulberry fruit (FEM) was evaluated for the antioxidant activity in vitro and the hemolysis in red blood cell (RBC) and lipid peroxidation in liver in vivo. Materials and Methods: Antioxidant activities in vitro were measured by quantifying its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, reducing power, and Fe2+-chelating ability. FEM inhibits hemolysis in RBCs and effects of lipid peroxidation in the liver were estimated. Results: The total content of flavonoid compounds was 187.23 mg of quercetin equivalents per grams dried material. In the in vitro assays, FEM demonstrated a strong antioxidant effect, especially in DPPH scavenging activity and reducing power. Mouse RBC hemolysis induced by H2O2was significantly inhibited by FEM in a dose- and time-dependent manner. The effects of FEM on lipid peroxidation in liver, mitochondria, and microsome were investigated. The percentage of inhibition at high concentration (100 μg/mL) of FEM was 45.51%, 39.36%, and 42.78% for liver, mitochondria, and microsomes, respectively. These results suggest that the FEM possesses a strong antioxidant activity both in vivo and in vitro.


How to cite this article:
Raman ST, Ganeshan AP, Chen C, Jin C, Li SH, Chen HJ, Gui Z. In vitro and In vivo antioxidant activity of flavonoid extracted from mulberry fruit (Morus alba L.).Phcog Mag 2016;12:128-133


How to cite this URL:
Raman ST, Ganeshan AP, Chen C, Jin C, Li SH, Chen HJ, Gui Z. In vitro and In vivo antioxidant activity of flavonoid extracted from mulberry fruit (Morus alba L.). Phcog Mag [serial online] 2016 [cited 2019 Dec 9 ];12:128-133
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=46;spage=128;epage=133;aulast=Raman;type=0