Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 45  |  Page : 86--95

Endoplasmic reticulum stress-induced apoptotic pathway and mitochondrial dysregulation in HeLa cells treated with dichloromethane extract of Dillenia suffruticosa


Wan Abd Ghani Wan Nor Hafiza1, Latifah Saiful Yazan2, Yin Sim Tor4, Jhi Biau Foo4, Nurdin Armania2, Heshu Sulaiman Rahman3 
1 Department of Biomedical Sciences, Faculty of Medicine and Health Science, University Putra Malaysia, 43400 UPM Serdang, Selangor; College of Medical Laboratory Technology, Institute for Medical Research, Jin Pahang, 50588 Kuala Lumpur, Malaysia
2 Department of Biomedical Sciences, Faculty of Medicine and Health Science; Laboratory of Molecular Biomedicine, Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
3 Department of Microbiology and Pathology, Faculty of Veterinary Medicine; UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Correspondence Address:
Latifah Saiful Yazan
Laboratory of Molecular Biomedicine, Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor
Malaysia

Ethyl acetate and dichloromethane extract of Dillenia suffruticosa (EADS and DCMDS, respectively) can be a potential anticancer agent. The effects of EADS and DCMDS on the growth of HeLa cervical cancer cells and the expression of apoptotic-related proteins had been investigated in vitro. Cytotoxicity of the extracts toward the cells was determined by 5-diphenyltetrazolium bromide assay, the effects on cell cycle progression and the mode of cell death were analyzed by flow cytometry technique, while the effects on apoptotic-related genes and proteins were evaluated by quantitative real-time polymerase chain reaction, and Western blot and enzyme-linked immunosorbent assay, respectively. Treatment with DCMDS inhibited (P < 0.05) proliferation and induced apoptosis in HeLa cells. The expression of cyclin B1 was downregulated that led to G2/M arrest in the cells after treatment with DCMDA. In summary, DCMDS induced apoptosis in HeLa cells via endoplasmic reticulum stress-induced apoptotic pathway and dysregulation of mitochondria. The data suggest the potential application of DCMDS in the treatment of cervical cancer.


How to cite this article:
Wan Nor Hafiza WA, Yazan LS, Tor YS, Foo JB, Armania N, Rahman HS. Endoplasmic reticulum stress-induced apoptotic pathway and mitochondrial dysregulation in HeLa cells treated with dichloromethane extract of Dillenia suffruticosa.Phcog Mag 2016;12:86-95


How to cite this URL:
Wan Nor Hafiza WA, Yazan LS, Tor YS, Foo JB, Armania N, Rahman HS. Endoplasmic reticulum stress-induced apoptotic pathway and mitochondrial dysregulation in HeLa cells treated with dichloromethane extract of Dillenia suffruticosa. Phcog Mag [serial online] 2016 [cited 2020 Aug 13 ];12:86-95
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=45;spage=86;epage=95;aulast=Wan;type=0