Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 45  |  Page : 70--74

Optimization of process parameters and kinetic model of enzymatic extraction of polyphenols from Lonicerae flos


Fansheng Kong1, Shujuan Yu2, Yongguang Bi3, Xiaojun Huang3, Mengqian Huang3 
1 College of Light Industry and Food Sciences, South China University of Technology; College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China
2 College of Light Industry and Food Sciences, South China University of Technology; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China
3 College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China

Correspondence Address:
Shujuan Yu
College of Light Industry and Food Sciences, South China University of Technology, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640
China

Objective: To optimize and verify the cellulase extraction of polyphenols from honeysuckle and provide a reference for enzymatic extracting polyphenols from honeysuckle. Materials and Methods: The uniform design was used According to Fick«SQ»s first law and kinetic model, fitting analysis of the dynamic process of enzymatic extracting polyphenols was conducted. Results: The optimum enzymatic extraction parameters for polyphenols from honeysuckle are found to be 80% (v/v) of alcohol, 35:1 (mL/g) of liquid-solid ratio, 80°C of extraction temperature, 8.5 of pH, 6.0 mg of enzyme levels, and 130 min of extraction time. Under the optimal conditions, the extraction rate of polyphenols was 3.03%. The kinetic experiments indicated kinetic equation C / CC0 had a good linear relationship with t even under the conditions of different levels of enzyme and temperature, which means fitting curve tallies well with the experimental values. Conclusion: The results of quantification showed that the results provide a reference for enzymatic extracting polyphenols from honeysuckle.


How to cite this article:
Kong F, Yu S, Bi Y, Huang X, Huang M. Optimization of process parameters and kinetic model of enzymatic extraction of polyphenols from Lonicerae flos.Phcog Mag 2016;12:70-74


How to cite this URL:
Kong F, Yu S, Bi Y, Huang X, Huang M. Optimization of process parameters and kinetic model of enzymatic extraction of polyphenols from Lonicerae flos. Phcog Mag [serial online] 2016 [cited 2020 Jan 29 ];12:70-74
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=45;spage=70;epage=74;aulast=Kong;type=0