Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 12  |  Issue : 45  |  Page : 4--8

An efficient high-performance liquid chromatography combined with electrospray ionization tandem mass spectrometry method to elaborate the changes of components between the raw and processed radix Aconitum kusnezoffii


Beibei Wang1, Jiaojiao Ji1, Shuang Zhao1, Jie Dong1, Peng Tan1, Shengsang Na2, Yonggang Liu1 
1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
2 Department of Traditional Mongolia Medicine, Inner Mongolia Medical University, Hohehot, China

Correspondence Address:
Yonggang Liu
Beijing University of Chinese Medicine, Beijing 100102
China

Background: Crude radix Aconitum kusnezoffii (RAK) has great toxicity. Traditional Chinese medicine practice proved that processing may decrease its toxicity. In our previous study, we had established a new method of RAK processing (Paozhi). However, the mechanism is yet not perfect. Objective: To explore the related mechanism of processing through comparing the chemical contents. Materials and Methods: A new processing method of RAK named stoving (Hong Zhi) was used. In particular, RAK was stored at 110°C for 8 h, and then high performance liquid chromatography combined with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS n ) was developed for the detection of the alkaloids of the crude and processed RAK decoction pieces. Results: Thirty components of the crude RAK were discovered, among which, 23 alkaloids were identified. Meanwhile, 23 ingredients were detected in the processed RAK decoction pieces, among which, 20 alkaloids were determined yet. By comparison, eight alkaloids were found in both crude and processed RAK decoction pieces, 15 alkaloids were not found in the crude RAK, however, 10 new constituents yield after processing, which are 10-OH-hypaconine, 10-OH-mesaconine, isomer of bullatine A, 14-benzoyl-10-OH-mesaconine, 14-benzoyl-10-OH-aconine, 14-benzoyl-10-OH-hypaconine, dehydrated aconitine, 14-benzoylaconine, chuanfumine, dehydrated mesaconitine. Conclusion: The present study showed that significant change of alkaloids was detected in RAK before and after processing. Among them, the highly toxic diester alkaloids decreased and the less toxic monoester alkaloids increased. Moreover, the concentration changes significantly. HPLC-ESI-MS n are Efficient to elaborate the mechanism of reduction of toxicity and enhancement efficacy after processing.


How to cite this article:
Wang B, Ji J, Zhao S, Dong J, Tan P, Na S, Liu Y. An efficient high-performance liquid chromatography combined with electrospray ionization tandem mass spectrometry method to elaborate the changes of components between the raw and processed radix Aconitum kusnezoffii.Phcog Mag 2016;12:4-8


How to cite this URL:
Wang B, Ji J, Zhao S, Dong J, Tan P, Na S, Liu Y. An efficient high-performance liquid chromatography combined with electrospray ionization tandem mass spectrometry method to elaborate the changes of components between the raw and processed radix Aconitum kusnezoffii. Phcog Mag [serial online] 2016 [cited 2019 Dec 11 ];12:4-8
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=45;spage=4;epage=8;aulast=Wang;type=0