Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 43  |  Page : 546--554

Microwave assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity


Dan Wang1, Bi-Bo Zhang1, Xiao-Xia Qu1, Feng Gao1, Min-Yong Yuan2 
1 Department of Medicinal Plants, Agronomy College, Sichuan Agricultural University, Wenjiang Region, Chengdu 611130, P. R. China
2 Department of Pharmacy, The First Affiliated Hospital of Chengdu Medical College, Xindu Region, Chengdu 610500, P. R. China

Correspondence Address:
Feng Gao
Department of Medicinal Plants, Agronomy College, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang Region, Chengdu 611130
P. R. China

Background: Microwave assisted reflux extraction of polysaccharides YPF-P from the famous Chinese traditional drug, Yupingfeng powder, optimization of extracting conditions and evaluation of their antioxidant activity were conducted in this study. Results: Single factor effect trends were achieved through yields and contends of YPF P obtained from different extracting conditions. Then through a three level, four variable Box Behnken design of response surface methodology adopting yield as response, the optimal conditions were determined as follows: Material/solvent ratio 1:23.37, microwave power 560 W, Extraction temperature 64°C, and extraction time 9.62 min. Under the optimal conditions, the YPF P extraction yield was 3.23%, and its content was detected as 38.52%. In antioxidant assays, the YPF P was tested to possess 1,1 diphenyl 2 picrylhydrazyl (DPPH) radical scavenging activities with an IC50 value of 0.262 mg/ml. In addition, YPF P was also proved to have relatively low ferric reducing antioxidant power (FRAP), compared to Vc, through FRAP assay. Conclusion: In the microwave assisted reflux extraction research, good YPF P yield was achieved from materials with relatively low YPF P content. And for the first time, both DPPH and FRAP assays were conducted on YPF P, which proved that the antioxidant activity of YPF P contributed to the functions of this medicine.


How to cite this article:
Wang D, Zhang BB, Qu XX, Gao F, Yuan MY. Microwave assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity.Phcog Mag 2015;11:546-554


How to cite this URL:
Wang D, Zhang BB, Qu XX, Gao F, Yuan MY. Microwave assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity. Phcog Mag [serial online] 2015 [cited 2019 Dec 7 ];11:546-554
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=43;spage=546;epage=554;aulast=Wang;type=0