Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 11  |  Issue : 42  |  Page : 161--165

Effects of the extract and glycoalkaloids of Solanum lycocarpum St. Hill on Giardia lamblia trophozoites


Gilmarcio Z Martins1, Raquel R. D. Moreira2, Cleopatra S Planeta4, Adélia E Almeida3, Jairo K Bastos4, Lígia Salgueiro5, Carlos Cavaleiro5, Maria do Céu Sousa5 
1 UNIFEB, University Center of the Educational Foundation of Barretos, Avenida Professor Roberto Monte Frade, Barretos, São Paulo state; Department of Principles Natural Actives and Toxicology, Faculty of Pharmaceutical Sciences, UNESP - Estadual Paulista University, Araraquara, São Paulo state, Brazil
2 Department of Principles Natural Actives and Toxicology, Faculty of Pharmaceutical Sciences, UNESP - Estadual Paulista University, Araraquara, São Paulo state, Brazil; Center of Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Azinhaga of Santa Comba, Health Science Campus, Coimbra, Portugal
3 Departament of Drugs and Medicines, School of Pharmaceutical Sciences, UNESP - Estadual Paulista University, Araraquara, São Paulo state, Brazil
4 Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, USP, Ribeirão Preto, São Paulo state, Brazil
5 Center of Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Azinhaga of Santa Comba, Health Science Campus, Coimbra; Center of Neuroscience and Cell Biology, University of Coimbra, Largo Marques de Pombal, University of Coimbra, Coimbra, Portugal

Correspondence Address:
Dr. Raquel R. D. Moreira
Department of Principles Natural Actives and Toxicology, Faculty of Pharmaceutical Sciences, UNESP-Estadual Paulista University, Rod. Araraquara-Jau, Km 1 S/N, CEP 14801-902, Araraquara, São Paulo state, Brazil

Background: Solanum lycocarpum has great importance for food and medicinal traditional use. Recently, it was also evidenced that extracts of S. lycocarpum St. Hill (Solanaceae) and its glycoalkaloids, solamargine (Sg) and solasonine (Sn), are active against flagellated protozoa. Objective: The aim was to assess the effects of the extract of S. lycocarpum and its glycoalkaloids, Sn, and Sg, on Giardia lamblia trophozoites. Materials and Methods: A crude extract (96% ethanol) (EB) of fruits of S. lycocarpum was prepared and fractionated by partition with 40% ethanol and n-hexane: Ethyl acetate. Glycoalkaloids, Sn, and Sg were recognized in the ethanol fraction (EF) and further isolated by column chromatography. EB, EF, the isolated Sn and Sg and a mixture (1:1) of both glycoalkaloids were tested on cultures of G. lamblia trophozoites and macrophages. Results: EB, EF and glycoalkaloids of S. lycocarpum showed activity against Giardia (95.0 < Inhibitory concentration 50 [IC 50 ] ≤120.3 μg/mL). The mixture of glycoalkaloids (1:1) was more active (IC 50 = 13.23 μg/mL) than each one individually, suggesting a synergic effect. Moreover, the mixture is nontoxic to macrophage cells. Conclusion: Results are optimistic concerning the anti-Giardia potential of the mixture Sn + Sg. Further studies, in vitro and in vivo, will be required to consolidate the usefulness of the mixture of Sn + Sg in view of a new therapeutic strategy for giardiasis.


How to cite this article:
Martins GZ, Moreira RR, Planeta CS, Almeida AE, Bastos JK, Salgueiro L, Cavaleiro C, do Céu Sousa M. Effects of the extract and glycoalkaloids of Solanum lycocarpum St. Hill on Giardia lamblia trophozoites.Phcog Mag 2015;11:161-165


How to cite this URL:
Martins GZ, Moreira RR, Planeta CS, Almeida AE, Bastos JK, Salgueiro L, Cavaleiro C, do Céu Sousa M. Effects of the extract and glycoalkaloids of Solanum lycocarpum St. Hill on Giardia lamblia trophozoites. Phcog Mag [serial online] 2015 [cited 2019 Nov 14 ];11:161-165
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=42;spage=161;epage=165;aulast=Martins;type=0