Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 39  |  Page : 519--523

Anticancer activity of flavane gallates isolated from Plicosepalus curviflorus


Ghada Ahmed Fawzy, Areej Mohammad Al-Taweel, Shagufta Perveen 
 Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia; Faculty of Pharmacy, Cairo University, Cairo, Egypt

Correspondence Address:
Ghada Ahmed Fawzy
College of Pharmacy, Department of Pharmacognosy, Ministry of Higher Education, King Saud University, Medical Studies and Sciences Sections, Riyadh 11495, P. O. Box 22452, Kingdom of Saudi Arabia

Background: Previous investigation of the methanol extract of Plicosepalus curviflorus leaves led to the isolation of two new flavane gallates (1, 2), together with other compounds including quercetin (3). The stems of P. curviflorus are used traditionally for the treatment of cancer in Yemen. Objective: The aim of this study was to evaluate the anticancer activity of the plant methanol extract as well as isolated compounds (1-3). Materials and Methods: The human cancer cell lines used were; MCF-7, HepG-2, HCT-116, Hep-2, HeLa and normal, Vero cell line using the Crystal Violet Staining method (CVS). Results: Quercetin (3) possessed the highest anticancer effect against all five cell lines (IC 50 ranging from 3.6 to 16.2 μg/ml). It was followed by 2S, 3R-3, 3′, 4′, 5, 7-pentahydroxyflavane-5-O-gallate (1) , with IC 50 ranging from 11.6 to 38.8 μg/ml. The weakest anticancer activity was given by 2S, 3R-3,3′,4′,5,5′,7-hexahydroxyflavane-3′,5-di-O-gallate (2) with IC 50 ranging from 39.8 to above 50 μg/ml, compared to vinblastine sulphate as reference drug. Colon, liver and breast cell lines seemed to be more sensitive to the tested compounds than the cervical and laryngeal cell lines. Concerning the cytotoxic effect on Vero cell line, the pentahydroxyflavane-5-O-gallate (1) showed the highest IC 50 ( 138.2 μg/ml), while quercetin exhibited the lowest IC 50 to Vero cells (30.5 μg/ml), compared to vinblastine sulphate as reference drug (IC 50: 39.7 μg/ml). Conclusion: The results suggest the possible use of compounds 1 and 3 as anticancer drugs especially against colon and liver cancers.


How to cite this article:
Fawzy GA, Al-Taweel AM, Perveen S. Anticancer activity of flavane gallates isolated from Plicosepalus curviflorus .Phcog Mag 2014;10:519-523


How to cite this URL:
Fawzy GA, Al-Taweel AM, Perveen S. Anticancer activity of flavane gallates isolated from Plicosepalus curviflorus . Phcog Mag [serial online] 2014 [cited 2020 Aug 10 ];10:519-523
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=39;spage=519;epage=523;aulast=Fawzy;type=0