Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 39  |  Page : 265--270

Rapid and undamaged analysis of crude and processed Radix Scrophulariae by Fourier transform infrared spectroscopy coupled with soft independent modeling of class analogy


Huiping Zhu1, Gang Cao2, Hao Cai4, Baochang Cai4, Jue Hu3 
1 The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
2 Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China
3 School of Basic Medical Sciences, Zhejiang Medical College, Hangzhou, P. R., China

Correspondence Address:
Gang Cao
Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, P. R
China

Objective: The main objective of this work is to determine the feasibility of identification of crude and processed Radix Scrophulariae using the Fourier transform infrared spectroscopy couple with soft independent modeling of class analogy (FT-IR-SIMCA). Materials and Methods: A total of 50 different crude Radix Scrophulariae was used to product processed ones. The spectra were acquired by FT-IR spectroscopy using a diffuse reflectance fiber optic probe. For the multivariate analysis, SIMCA was used. Results showed that FT-IR-SIMCA was useful to discriminate the processed Radix Scrophulariae samples from crude samples. These samples could be successfully classified by SIMCA. Results: In all cases, the recognition and rejection rates were 97.8% and 100%, respectively. When testing with the blind sample that was picked out from the chosen samples, the accuracy was up to 90%. Conclusion: It means that the methodology is capable of accurately separating processed Radix Scrophulariae from crude samples.


How to cite this article:
Zhu H, Cao G, Cai H, Cai B, Hu J. Rapid and undamaged analysis of crude and processed Radix Scrophulariae by Fourier transform infrared spectroscopy coupled with soft independent modeling of class analogy.Phcog Mag 2014;10:265-270


How to cite this URL:
Zhu H, Cao G, Cai H, Cai B, Hu J. Rapid and undamaged analysis of crude and processed Radix Scrophulariae by Fourier transform infrared spectroscopy coupled with soft independent modeling of class analogy. Phcog Mag [serial online] 2014 [cited 2020 Jan 26 ];10:265-270
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=39;spage=265;epage=270;aulast=Zhu;type=0