Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 38  |  Page : 256--263

Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid chromatography-diode array detection


Bo-Ra Yun1, Jin Bae Weon1, Jiwoo Lee1, Min Rye Eom1, Choong Je Ma2 
1 Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea
2 Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University; Research Institute of Biotechnology, Kangwon National University, Chuncheon, Republic of Korea

Correspondence Address:
Choong Je Ma
Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Hyoja 2 Dong, Chuncheon 200 701
Republic of Korea

Background: Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. Objective: A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- hydroxymethylfurfural, mangiferin, paeoniflorin, nodakenin, naringin, hesperidin, decursinol, berberine, glycyrrhizin, atractylenolide III and decursin, in JEGH. Materials and Methods: The separation was conducted on Shishedo C 18 column with gradient elution of 0.1% trifluoroacetic acid-acetonitrile. Detection of wavelength was set at 205, 250, 280 and 330 nm. Results: The developed analysis showed a good linearity (R2 >0.9997). The range of limit of detection and limit of quantification were observed from 0.04 to 0.43 and from 0.11 to 1.30, respectively. The intra- and inter-day test relative standard deviations (RSD) were less than 3% and the accuracy was 95.98-108.44%. The recoveries were between 92.75% and 109.19% and RSD range of recoveries was measured from 0.52% to 2.78%. Conclusion: This HPLC-DAD method can be successfully applied for simultaneous determination of 11 major compounds in JEGH samples.


How to cite this article:
Yun BR, Weon JB, Lee J, Eom MR, Ma CJ. Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid chromatography-diode array detection.Phcog Mag 2014;10:256-263


How to cite this URL:
Yun BR, Weon JB, Lee J, Eom MR, Ma CJ. Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid chromatography-diode array detection. Phcog Mag [serial online] 2014 [cited 2019 Dec 8 ];10:256-263
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=38;spage=256;epage=263;aulast=Yun;type=0