Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 37  |  Page : 50--56

Antidepressant-like activity of Chaihu-Shugan-San aqueous extract in rats and its possible mechanism


Sue Wang1, Suiyu Hu1, Chunhu Zhang1, Juan Qiu2, Yunhui Li1 
1 Department of Integrative Medicine, Institute of Integrative Medicine, Xiangya Hospital, Central South University; Department of Integrative Medicine, Key Unit of State Administration of Traditional Chinese Medicine of the People's Republic of China, Changsha, Hunan, China
2 Department of Nuclear Medicine, Xiangya 3rd Hospital, Central South University, Changsha, Hunan, China

Correspondence Address:
Sue Wang
Institute of Integrative Medicine, Xiangya Hospital, Central South University, No. 87, Xiangya Road, Changsha, Hunan 410 008
China

Background: Chaihu-Shugan-San (CHSGS), a traditional Chinese medicinal formula, is commonly used for the treatment of depression in China. However, the molecular mechanism underlying its antidepressant action is unknown. Objective: The objective of this study is to evaluate the antidepressant-like effects of CHSGS and further explore the possible molecular mechanism implicated in its actions. Materials and Methods: The rats were randomly divided into four groups: The normal control group, the model control group, the CHSGS group and the fluoxetine control group. The antidepressant-like effects of CHSGS aqueous extract were assessed in rats exposed to chronic mild stress (CMS) using the open-field test and sucrose water consumption test, its underlying mechanism of anti-depression was explored by determining the effect of CHSGS on the extracellular signal-regulated kinase (ERK) and phospho-ERK (P-ERK) in the hippocampus using western blot. The aqueous extract of CHSGS at a dose of standard (5.9 g/kg·d) was administered intragastrically for 14 days during the CMS model while the fluoxetine control group was given at the same time using fluoxetine hydrochloride (1.8 mg/kg·d). Results: The stressed rats demonstrated decreased locomotor activity in open field test and reduction in sucrose consumption and decreased levels of P-ERK1/2 and the ratio of P-ERK1/2 to total ERK1/2 in the hippocampus. CHSGS alleviated the depressive-like behaviors and increased levels of P-ERK1/2 and the ratio of P-ERK1/2 to total ERK1/2 in stressed rats as well as fluoxetine. Conclusion: In summary, these results suggest that CHSGS aqueous extract possesses an antidepressant-like activity in CMS induced depression model rats, which might be mediated, at least in part, by reversing the stress-induced disruption of ERK activity.


How to cite this article:
Wang S, Hu S, Zhang C, Qiu J, Li Y. Antidepressant-like activity of Chaihu-Shugan-San aqueous extract in rats and its possible mechanism.Phcog Mag 2014;10:50-56


How to cite this URL:
Wang S, Hu S, Zhang C, Qiu J, Li Y. Antidepressant-like activity of Chaihu-Shugan-San aqueous extract in rats and its possible mechanism. Phcog Mag [serial online] 2014 [cited 2019 Nov 13 ];10:50-56
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=37;spage=50;epage=56;aulast=Wang;type=0