Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 37  |  Page : 27--33

Heartwood extract of Acacia catechu induces apoptosis in human breast carcinoma by altering bax/bcl-2 ratio


Nikhil Baban Ghate, Bibhabasu Hazra, Rhitajit Sarkar, Nripendranath Mandal 
 Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India

Correspondence Address:
Nripendranath Mandal
Division of Molecular Medicine, Bose Institute, P-1/12 C. I. T. Scheme VII M, Kolkata - 700 054, West Bengal
India

Background: The heartwood extract of A. catechu, called pale catechu or źDQ╗KathaźDQ╗ in Hindi has been widely used in traditional Indian medicinal system. Although various pharmacological properties of this plant had been reported previously, only a few were concerned with the anticancer activity of this plant. Objective: The objective was to assess the in vitro anticancer and apoptosis inducing effect of 70% methanolic extract of źDQ╗KathaźDQ╗ (ACME) on human breast adenocarcinoma cell line (MCF-7). Materials and Methods: MCF-7 cell line was treated with increasing concentrations of ACME and cell viability was calculated. Flow cytometric methods were used to confirm the apoptosis promoting role of ACME. Morphological changes were then analysed using confocal microscopy. Western blotting was then performed to investigate the expression of apoptogenic proteins and to analyse the activation of caspases. Results: ACME showed significant cytotoxicity to MCF-7 cells with an IC 50 value of 288.85 ± 25.79 μg/ml. Flow cytometric analysis and morphological studies confirmed that ACME is able to induce apoptosis in MCF-7 cells. Furthermore, immunoblot results suggested the pathway of apoptosis induction by increasing Bax/Bcl-2 ratio which results in the activation of caspase-cascade and ultimately leads to the cleavage of Poly adeno ribose polymerase (PARP). Conclusion: These results provide the evidence that ACME is able to inhibit the proliferation of MCF-7 cells by inducing apoptosis through intrinsic pathway.


How to cite this article:
Ghate NB, Hazra B, Sarkar R, Mandal N. Heartwood extract of Acacia catechu induces apoptosis in human breast carcinoma by altering bax/bcl-2 ratio.Phcog Mag 2014;10:27-33


How to cite this URL:
Ghate NB, Hazra B, Sarkar R, Mandal N. Heartwood extract of Acacia catechu induces apoptosis in human breast carcinoma by altering bax/bcl-2 ratio. Phcog Mag [serial online] 2014 [cited 2019 Aug 25 ];10:27-33
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=37;spage=27;epage=33;aulast=Ghate;type=0