Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 9  |  Issue : 36  |  Page : 331--337

Identification of medical plants of 24 Ardisia species from China using the matK genetic marker


Yimei Liu1, Ke Wang1, Zhen Liu1, Kun Luo2, Shilin Chen2, Keli Chen1 
1 Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China
2 Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

Correspondence Address:
Keli Chen
Hubei University of Chinese Medicine, Wuhan 430065
China

Background: Ardisia is a group of famous herbs in China, which has been used as medical plants for more than 900 years. However, the species from the genus are so analogous that it is difficult to discriminate them just by morphological characteristics. DNA barcoding is a new technique that uses a short and standard fragment of DNA sequences to identify species. Objective: Choose a suitable DNA marker to authenticate Ardisia species. Materials and Methods: Four markers (psbA-trnH, internal transcribed spacer 2 [ITS2], rbcL, matK) were tested on 54 samples of 24 species from genus Ardisia. The success rates of polymerase chain reaction amplification and sequencing, differential intra- and inter-specific divergences, DNA barcoding gap and identification efficiency were used to evaluate the discrimination ability. Results : The results indicate that matK has the highest interspecific divergence and significant differences between inter- and intra-specific divergences, whereas psbA-trnH, ITS2 and rbcL have much lower divergence values. Matk possessed the highest species identification efficiency at 98.1% by basic local alignment search tool 1 [BLAST1], method and 91.7% by the nearest distance method. Conclusion: The matK region is a promising DNA barcode for the genus Ardisia.


How to cite this article:
Liu Y, Wang K, Liu Z, Luo K, Chen S, Chen K. Identification of medical plants of 24 Ardisia species from China using the matK genetic marker.Phcog Mag 2013;9:331-337


How to cite this URL:
Liu Y, Wang K, Liu Z, Luo K, Chen S, Chen K. Identification of medical plants of 24 Ardisia species from China using the matK genetic marker. Phcog Mag [serial online] 2013 [cited 2019 Nov 13 ];9:331-337
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2013;volume=9;issue=36;spage=331;epage=337;aulast=Liu;type=0