Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2012  |  Volume : 8  |  Issue : 30  |  Page : 111--115

Effect of Agrobacterium rhizogenes and elicitation on the asiaticoside production in cell cultures of Centella asiatica


Komar Ruslan1, Anggrahaeni Dewi Selfitri1, Shella A Bulan1, Yaya Rukayadi2, Elfahmi1 
1 Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
2 Biopharmaca Research Center, Bogor Agricultural University, Bogor, Indonesia

Correspondence Address:
Elfahmi
Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Jl. Ganesha 10 Bandung 40132
Indonesia

Background: Centella asiatica (L.) Urb. (Apiaceae) is an important medicinal plant, and it has been using to prepare herbal medicines. The compounds responsible for the biological activity of C. asiatica are triterpenoids such as asiaticoside. Asiaticoside is also important as a marker for standardization of C. asiatica. Due to the low content, there is a need to enhance the production of asiaticoside of C. asiatica. The biotechnological approach is one of the methods that can be used to enhance its production. Objectives: This study was designed to enhance the production of asiaticoside from C. asiatica using A. rhizogenes and elicitation experiments. Materials and Methods : Callus cultures were initiated using Murashige and Skoog (MS) medium supplemented with 1.0 mg/L indole-3-acetic acid (IAA) and 1.0 mg/L 6-benzylaminopurin (BAP). All media were supplemented with 4% (w/w) sucrose and solidified with 0.9% agar. Elicitations were done using pectin, methyl jasmonate, and Cu 2+ ions. Transformed hairy root cultures were performed using A. rhizogenes. Results: Callus culture of C. asiatica was successfully initiated. Enhancement of the production of asiaticoside in the callus culture by elicitors pectin was up to 31%; methyl jasmonate (50 ΅M) in cell suspension cultures at day 14 was up to 171% compared to explant and 494% compared to control callus; copper ion (25 ΅M) at day 21 was up to 144% compared to explant, and 676% compared to control cell suspension cultures. While enhancement by genetic transformation using A. rhizogenes was 166-172% compare to untransformed roots Conclusion: Elicitation and genetically transformed hairy root cultures of C. asiatica produced asiaticoside up to 172% higher than untreated callus.


How to cite this article:
Ruslan K, Selfitri AD, Bulan SA, Rukayadi Y, Elfahmi. Effect of Agrobacterium rhizogenes and elicitation on the asiaticoside production in cell cultures of Centella asiatica.Phcog Mag 2012;8:111-115


How to cite this URL:
Ruslan K, Selfitri AD, Bulan SA, Rukayadi Y, Elfahmi. Effect of Agrobacterium rhizogenes and elicitation on the asiaticoside production in cell cultures of Centella asiatica. Phcog Mag [serial online] 2012 [cited 2019 Oct 22 ];8:111-115
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2012;volume=8;issue=30;spage=111;epage=115;aulast=Ruslan;type=0