Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2011  |  Volume : 7  |  Issue : 27  |  Page : 243--247

Effect of cleistanthin A and B on adrenergic and cholinergic receptors


S Parasuraman, R Raveendran 
 Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India

Correspondence Address:
S Parasuraman
Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry
India

Objective: The aim was to study the in vitro and in silico interactions of cleistanthin A and B on the adrenergic and cholinergic receptors using isolated animal tissues and bioinformatics tools. Materials and Methods: The alpha adrenergic receptor activities of cleistanthin A and B were studied in vitro using a guinea pig vas deferens preparation. The beta adrenergic receptor activities of cleistanthin A and B on an isolated rat heart were studied in vitro using a modified Langendorff apparatus. The effects of cleistanthin A and B on the nicotinic and muscarinic cholinergic receptors were studied in vitro using rabbit vas deferens and rabbit jejunum, respectively. All the drug responses were recorded using a data acquisition system through a variable force transducer. The receptor-ligand interactions of cleistanthin A and B with adrenergic and cholinergic receptor proteins were determined using the ArgusLab molecular modeling and drug docking program. Results: Cleistanthin A and B significantly inhibited the actions of the alpha adrenergic receptor and the nicotinic cholinergic receptor. Cleistanthin A and B shifted the dose-response curve to the right with an increased EC50 value of phenylephrine and acetylcholine. Both cleistanthin A and B did not have any significant effect on the beta adrenergic and muscarinic cholinergic receptors. Conclusion: Cleistanthin A and B block the alpha adrenergic and nicotinic cholinergic receptors, but these compounds do not interact at all with the beta adrenergic and muscarinic cholinergic receptors.


How to cite this article:
Parasuraman S, Raveendran R. Effect of cleistanthin A and B on adrenergic and cholinergic receptors.Phcog Mag 2011;7:243-247


How to cite this URL:
Parasuraman S, Raveendran R. Effect of cleistanthin A and B on adrenergic and cholinergic receptors. Phcog Mag [serial online] 2011 [cited 2020 Aug 10 ];7:243-247
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2011;volume=7;issue=27;spage=243;epage=247;aulast=Parasuraman;type=0