Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2011  |  Volume : 7  |  Issue : 27  |  Page : 186--192

Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze


Xinhui Tang1, Lifang Yan2, Jing Gao4, Haiyan Ge4, Haidong Yang4, Na Lin4 
1 Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002, People's Republic of China
2 Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002; School of Pharmacy, Jiangsu University, Zhenjiang - 212013, People's Republic of China

Correspondence Address:
Jing Gao
School of Pharmacy, Jiangsu University, Zhenjiang, 212013
People«SQ»s Republic of China

Objective: To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP) Materials and Methods : One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2«SQ»-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical assays were established to measure the antioxidant capacity of the LSEP in vitro. Results: According to analysis, extraction temperature significantly affected extraction yield. The optimum extraction conditions for LSEP were as follows: extraction temperature, 95°C; ultrasonic time 50 minutes; and dosage liquor ratio, 1: 12. Under these conditions, the experimental yield of crude LSEP was 12.80±0.19% which was well matched with the predicted models. The antioxidant capacity data suggested that LSEP has strong antioxidant activity. Conclusion: One-singer factor and RSM were used to extract of LSEP are simple and feasible and LSEP could be developed as a nutraceutical agent for itsstrong antioxidant activity.


How to cite this article:
Tang X, Yan L, Gao J, Ge H, Yang H, Lin N. Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze.Phcog Mag 2011;7:186-192


How to cite this URL:
Tang X, Yan L, Gao J, Ge H, Yang H, Lin N. Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze. Phcog Mag [serial online] 2011 [cited 2019 Dec 6 ];7:186-192
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2011;volume=7;issue=27;spage=186;epage=192;aulast=Tang;type=0