Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2011  |  Volume : 7  |  Issue : 26  |  Page : 101--108

Isolation and identification of phenolic compounds from Gynura divaricata leaves


Chunpeng Wan1, Yanying Yu2, Shouran Zhou3, Shuge Tian4, Shuwen Cao5 
1 State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang-330047, Jiangxi, China
2 Department of Chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China
3 Jiangxi University of Traditional Chinese Medicine, Nanchang - 330 006, Jiangxi, China
4 Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi - 830 011, Xinjiang, China
5 State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang-330047, Jiangxi; Department of Chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China

Correspondence Address:
Shuwen Cao
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang - 330 047, Jiangxi
China

Background: Phenolic constituents were the principle bioactivity compounds exist in Gynura divaricata, little phenolic compounds were reported from the plant previously. Materials and Methods: 60% ethanol extract from the leaves of Gynura divaricata were isolated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Additionally, a high-performance liquid chromatography-diode array detector-electrospray ionization-mass (HPLC-DAD-ESI-MS) analytical method was developed to identify some minor constituents in the n-butanol fraction of the ethanol extract of Gynura divaricata. Results: Six flavonols and one Dicaffeoylquinic acid were isolated from the leaves of Gynura divaricata, and these compounds were identified as follows: quercetin (1), kaempferol (2), kaempferol-3-O-β-D-glucopyranoside (3), quercetin-3-O-rutinoside (4), kaempferol-3,7-di-O-β-D-glucopyranoside (5), kaempferol-3-O-rutinoside-7-O-β-D-glucopyranoside (6), and 3,5-dicaffeoylquinic acid (7). A total of 13 compounds, including 9 flavonol glycosides and 4 phenolic acids, were tentatively identified by comparing their retention time (RT), UV, and MS spectrum values with those that had been identified and the published data. Conclusion: This was the first time to use the HPLC-DAD-ESI-MS method to identify the phytochemicals of the genera Gynura. Moreover, compounds (6) and (7) have been isolated for the first time from the genus Gynura.


How to cite this article:
Wan C, Yu Y, Zhou S, Tian S, Cao S. Isolation and identification of phenolic compounds from Gynura divaricata leaves.Phcog Mag 2011;7:101-108


How to cite this URL:
Wan C, Yu Y, Zhou S, Tian S, Cao S. Isolation and identification of phenolic compounds from Gynura divaricata leaves. Phcog Mag [serial online] 2011 [cited 2019 Nov 17 ];7:101-108
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2011;volume=7;issue=26;spage=101;epage=108;aulast=Wan;type=0