Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2011  |  Volume : 7  |  Issue : 25  |  Page : 40--45

Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures


Chunpeng Wan1, Yanying Yu2, Shouran Zhou3, Wei Liu1, Shuge Tian4, Shuwen Cao5 
1 State Key Laboratory of Food Science & Technology, Nanchang University, Nanchang - 330 047, Jiangxi, China
2 Department of chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China
3 Jiangxi University of Traditional Chinese Medicine, Nanchang - 330 006, Jiangxi, China
4 Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi - 830 011, Xinjiang, China
5 State Key Laboratory of Food Science & Technology, Nanchang University, Nanchang - 330 047, Jiangxi; Department of chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China

Correspondence Address:
Shuwen Cao
State Key Laboratory of Food Science & Technology, Nanchang University, Nanchang - 330 047, Jiangxi
China

Background: Extraction temperature influences the total phenolic content (TPC), total flavonoid content (TFC) of medicinal plant extracts to a great extend. TPC and TFC are the principle activity constituents present in the plant. The effects of extraction temperature on TPC, TFC and free radical-scavenging capacity of Gynura divaricata leaf extracts are worth to study. Materials and Methods: Folin-Ciocalteu and aluminum chloride colorimetric assay were used to determine the TPC and TFC of Gynura divaricata leaf extracts at different temperatures. The antioxidant and free radical-scavenging activity were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and phosphomolybdenum methods. Results: TPC and TFC were significantly elevated with increasing extraction temperature (from 40°C to 100°C). However, TPC and TFC were not significantly different (P > 0.05) at the extraction temperatures 90°C and 100°C. Also, the extracts obtained at a higher temperature exhibited a significant free radical-scavenging activity compared with extraction at lower temperatures (P < 0.05). The TPCs (13.95-36.68 mg gallic acid equivalent/g dry material) were highly correlated with DPPH (R2 = 0.9229), ABTS (R2 = 0.9951) free radical-scavenging capacity, and total antioxidant activity (R2 = 0.9872) evaluated by phosphomolybdenum method. Conclusion: The TPC and TFC of G. divaricata leaf was significantly influenced by the extraction temperatures, which were the main antioxidant constituents present in the G. divaricata plant.


How to cite this article:
Wan C, Yu Y, Zhou S, Liu W, Tian S, Cao S. Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures.Phcog Mag 2011;7:40-45


How to cite this URL:
Wan C, Yu Y, Zhou S, Liu W, Tian S, Cao S. Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures. Phcog Mag [serial online] 2011 [cited 2019 Dec 11 ];7:40-45
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2011;volume=7;issue=25;spage=40;epage=45;aulast=Wan;type=0