Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2010  |  Volume : 6  |  Issue : 24  |  Page : 286--292

Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton


Wei Kun-Hua, Gao Shan-Lin, Huang He-Ping 
 Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu - 211 198, People's Republic of China

Correspondence Address:
Gao Shan-Lin
Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu - 211 198
People«SQ»s Republic of China

Background: Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication. Objective: To establish and optimize the rapid propagation technology of S. flavescens and to generate and characterize polyploid plants of S. flavescens. Materials and Methods: The different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to establish and screen the optimal rapid propagation technology of S. flavescens by orthogonal test; 0.2% colchicine solution was used to induce polyploid plants and the induced buds were identified by root-tip chromosome determination and stomatal apparatus observation. Results: A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog medium (MS; 1962) supplemented with 1.4-1.6 mg/l 6-benzylaminopurine (BAP) and 0.3 mg/l indole-3-acetic acid (IAA). More than 50 lines of autotetraploid plants were obtained. The chromosome number of the autotetraploid plantlet was 2n = 4x = 36. All tetraploid plants showed typical polyploid characteristics. Conclusion: Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.


How to cite this article:
Kun-Hua W, Shan-Lin G, He-Ping H. Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.Phcog Mag 2010;6:286-292


How to cite this URL:
Kun-Hua W, Shan-Lin G, He-Ping H. Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton. Phcog Mag [serial online] 2010 [cited 2020 Aug 13 ];6:286-292
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2010;volume=6;issue=24;spage=286;epage=292;aulast=Kun-Hua;type=0