Pharmacognosy Magazine

RESEARCH ARTICLE
Year
: 2009  |  Volume : 5  |  Issue : 20  |  Page : 336--342

Agrobacterium rhizogenes Mediated Genetic transformation of Abrus precatorius L


Vijai Singh Karwasara, VK Dixit 
 Biotechnology Research Laboratory, Department of Pharmaceutical Sciences, Dr. H.S. Gour University, Sagar-470003 (M.P.), India

Correspondence Address:
V K Dixit
Biotechnology Research Laboratory, Department of Pharmaceutical Sciences, Dr. H.S. Gour University, Sagar-470003 (M.P.)
India

Abrus precatorius L. known as Indian liquorice is a common deciduous vine containing sweet principle compound known as glycyrrhizin. Hence it can be used as a very good substitute for Liquorice. Genetic transformation has proved to be an effective way to enhance secondary metabolites in plant cell cultures. The transformation of Abrus precatorius L. mediated by Agrobacterium rhizogenes was studied using three bacterial strains i.e. MTCC 532, MTCCC 2364 and NCIM 5140. Putative hairy roots were obtained after the transformation. The effects of bacterial strains, bacterial concentration, acetosyringone and co-cultivation pH on transformation of Abrus were investigated. Co-cultivation with Strain MTCC 532 for 2 days with 100 µmol L -1 acetosyringone at pH 6.5 provided the optimal conditions under which transformation frequency approached 84%.


How to cite this article:
Karwasara VS, Dixit V K. Agrobacterium rhizogenes Mediated Genetic transformation of Abrus precatorius L.Phcog Mag 2009;5:336-342


How to cite this URL:
Karwasara VS, Dixit V K. Agrobacterium rhizogenes Mediated Genetic transformation of Abrus precatorius L. Phcog Mag [serial online] 2009 [cited 2020 Jul 8 ];5:336-342
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2009;volume=5;issue=20;spage=336;epage=342;aulast=Karwasara;type=0