Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2020  |  Volume : 16  |  Issue : 70  |  Page : 360-370

Althea rosea seed extract ameliorates 1,2-dimethylhydrazine induced preneoplastic lesions in mouse model of colon cancer by modulating oxidative stress and inflammation


1 Department of Biochemistry, Basic Medical Science, South Campus, Panjab University, Chandigarh, India
2 Department of Biochemistry, Basic Medical Science, South Campus, Panjab University, Chandigarh; Block-J, Pharmacology and Toxicology Laboratory, CSIR-IHBT, Palampur, Himachal Pradesh, India
3 Department of Immunopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Navneet Agnihotri
Department of Biochemistry, BMS Block-II, Sector-25, South Campus, Panjab University, Chandigarh - 160 014
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pm.pm_559_19

Rights and Permissions

Background: Phytochemicals with strong antioxidant and anti-inflammatory properties are known to modulate the process of carcinogenesis. Althea rosea (AR) is an ornamental plant and is an integral part of traditional medicine for curing a wide range of inflammatory disorders such as asthma, inflammatory bowel diseases, and arthritis. Therefore, its potential as a chemopreventive agent in cancer needs to be evaluated using an appropriate animal model. Materials and Methods: In this study, different in vitro assays including total phenolic content, 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate), and ferric reducing antioxidant power were used to evaluate the antioxidant capacity of AR seed extract. In addition, in vivo study at two different doses, i.e., 100 and 200 mg/kg body weight, was also conducted to analyze the chemopreventive potential of AR seed extract. The chemopreventive efficacy of AR seed extract was assessed by analysis of aberrant crypt foci (ACF), goblet cells/crypt, apoptotic index, and nuclear factor-kappa B (NF-κB) signaling pathway. Results: The results of in vitro assays suggested that AR seed extract exhibits a strong antioxidant potential. Administration of AR seed extract to 1,2-dimethylhydrazine group animals resulted in a marked reduction in ACF number, lymphocytic infiltration, and erosion of mucin layer from the intestinal epithelium. AR seed extract induced apoptosis in colonocytes as evident from the analysis of cleaved caspase-3, Bcl-2, and poly (ADP-ribose) polymerase 1/2 expression. Furthermore, treatment with AR seed extract inhibited the expression of NF-κB, a central mediator of chronic inflammation. The AR seed extract also ameliorated the damaging effects of oxidative stress by decreasing free radical generation and increasing the levels of enzymatic and non-enzymatic antioxidants. Conclusion: Taken together, these findings emphasized that AR seed extract could be considered as promising natural chemopreventive against colon carcinogenesis and should be further evaluated for the identification of active principle(s).


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed251    
    Printed2    
    Emailed0    
    PDF Downloaded21    
    Comments [Add]    

Recommend this journal